Western blot shows lysates of A172 human glioblastoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, BaF3 mouse pro-B cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 ...read more
NCK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human NCK1 Monoclonal Antibody (Catalog # MAB7008) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Protein A or G purified from hybridoma culture supernatant
Sterile PBS to a final concentration of 0.5 mg/mL.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for NCK1 Antibody (714506)
cytoplasmic protein NCK1
NCK adaptor protein 1melanoma NCK protein
NCKNCK tyrosine kinase
non-catalytic region of tyrosine kinase
SH2/SH3 adaptor protein NCK-alpha
NCK1 (Non-catalytic region of tyrosine kinase 1; also Sh2/SH3 adaptor protein NCK-alpha ) is a 46-48 kDa member of the Nck family of adaptor proteins. NCK1 is ubiquitously expressed and may be found in both cytoplasm and nucleus. In the cytoplasm, it connects tyrosine kinases to actin reorganization which, in T cells, involves SLP-76 and VAV1. Upon DNA damage, NCK1 binds SOCS7 and is translocated into the nucleus where it participates in p53 phosphorylation and cell cycle arrest. Human NCK1 is 377 amino acids (aa) in length. It contains three N-terminal SH3 domains (aa 2-252) that mediate binding to Pro-rich regions of cytoplasmic proteins, and a C-terminal SH2 domain (aa 282-376) that binds to phosphorylated proteins or receptors. There are at least three utilized Ser, and one utilized Tyr phosphorylation sites. There are also three potential splice variants. One shows a 12 aa substitution for aa 1-76, a second possesses an 11 aa substitution for aa 77‑377, and a third contains a 37 aa substitution for aa 134-377. Full-length NCK1 shares 99% aa identity with mouse NCK1.
MAB7008 detects mouse and rat NCK1 in Western Blots. Cross-reactivity with mouse and rat samples in immunocytochemistry has not been tested.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our NCK1 Antibody (714506) and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.