NCAM-1/CD56 Antibody (301021) [Unconjugated]

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuropil. Protocol available in COMET™ Panel Builder." > DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuropil. Protocol available in COMET™ Panel Builder." title="NCAM-1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Mouse Anti-Human NCAM-1/CD56, Monoclonal Antibody (Catalog # MAB24081) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuropil. Protocol available in COMET™ Panel Builder." />

NCAM-1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Mouse Anti-Human NCAM-1/CD56, Monoclonal Antibody (Catalog # MAB24081) at 25ug/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NCAM‑1/CD56 at approximately 105-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western blot shows lysates of human brain (cerebellum and hypothalamus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol. " > MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol. " title=" Human PBMCs were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (FAB1850P). Staining was performed using our Staining Membrane-associated Proteins protocol. " />

Human PBMCs were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (<a class=

CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " > CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " title="Human NK cells were expanded from PBMCs using Cloudz Human NK Cell Expansion Kit (CLD004) and were stained with either (A) Mouse Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24081) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-HumanCD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol. " />

Human NK cells were expanded from PBMCs using Cloudz Human NK Cell Expansion Kit (<a class=

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the neuropil. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the neuropil. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="NCAM‑1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) at 25 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the neuropil. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

NCAM‑1/CD56 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human NCAM‑1/CD56 Monoclonal Antibody (Catalog # MAB24081) at 25 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready, mIF
• Clone: 301021
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NCAM-1/CD56 Antibody (301021) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NCAM-1/CD56
  Leu20-Pro603 and Glu636-Asn741
  Accession # NP_001070150
• Specificity: Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCAM-1/CD56 Antibody (301021) [Unconjugated]

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
4. Dickson, G. et al. (1987) Cell 50:1119.
5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
13. Muller, D. et al. (1996) Neuron 17:413.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCAM-1/CD56 Antibody (MAB24081)(12)

Publications using MAB24081

• Aouimeur, I;Sagnial, T;Travers, G;Coulomb, L;Perrache, C;Maurin, C;Ninotta, S;Moisan, A;Bui-Quoc, E;Geslain, G;Thuret, JY;Gain, P;ThureT, G;He, Z; Key donor corneal factors governing successful expansion of human corneal endothelial cells Scientific reports 2026-05-11 [PMID: 42115358] (Immunocytochemistry, Human)
• Travers, G;Coulomb, L;Aouimeur, I;He, Z;Bonnet, G;Ollier, E;Gavet, Y;Moisan, A;Gain, P;Thuret, G;Maurin, C; Investigating the role of molecular coating in human corneal endothelial cell primary culture using artificial intelligence-driven image analysis Scientific reports 2025-08-25 [PMID: 40854928] (Immunocytochemistry, Human)
• Aouimeur, I;Sagnial, T;Coulomb, L;Maurin, C;Thomas, J;Forestier, P;Ninotta, S;Perrache, C;Forest, F;Gain, P;Thuret, G;He, Z; Investigating the Role of TGF-? Signaling Pathways in Human Corneal Endothelial Cell Primary Culture Cells 2023-06-14 [PMID: 37371094] (ICC, Human)
• Eun Ah Ye, Ho Seok Chung, Yoonkyung Park, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim et al. Induction of Corneal Endothelial-like Cells from Mesenchymal Stem Cells of the Umbilical Cord International Journal of Molecular Sciences 12/6/2022 [PMID: 36499735]
• Eun Ah Ye, Ho Seok Chung, Yoonkyung Park, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim et al. Induction of Corneal Endothelial-like Cells from Mesenchymal Stem Cells of the Umbilical Cord International Journal of Molecular Sciences 2022-12-06 [PMID: 36499735] (Immunocytochemistry, Human)
• Guoxu Zheng, Zhangyan Guo, Weimiao Li, Wenjin Xi, Baile Zuo, Rui Zhang et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab Signal Transduction and Targeted Therapy 6/23/2021 [PMID: 34158475]
• X Wang, M Lu, X Tian, Y Ren, Y Li, M Xiang, S Chen Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey Stem Cell Res Ther, 2020-08-03;11(1):334. 2020-08-03 [PMID: 32746912] (Human)
• Sylvie Berthier, Louis Larrouquère, Pierre Champelovier, Edwige Col, Christine Lefebvre, Cécile Cottet-Rouselle et al. A New Patient-Derived Metastatic Glioblastoma Cell Line: Characterisation and Response to Sodium Selenite Anticancer Agent Cancers (Basel) 12/21/2018 [PMID: 30583471]
• T Frenzel, B Hoffmann, R Schmitz, A Bethge, U Schumacher, G Wedemann Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model PLoS ONE, 2017-11-06;12(11):e0187144. 2017-11-06 [PMID: 29107953] (IHC-P, Mouse)
• Zhiguo He, Fabien Forest, Philippe Gain, Damien Rageade, Aurélien Bernard, Sophie Acquart et al. 3D map of the human corneal endothelial cell Scientific Reports 7/6/2016 [PMID: 27381832]
• Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M Selectins mediate small cell lung cancer systemic metastasis. PLoS ONE, 2014-04-03;9(4):e92327. 2014-04-03 [PMID: 24699516] (Flow Cytometry, Human)
• Theriault ME, Pare MÈ, Maltais F, Debigare R Satellite cells senescence in limb muscle of severe patients with COPD. PLoS ONE, 2012-06-13;7(6):e39124. 2012-06-13 [PMID: 22720047] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: NCAM1
• Uniprot:
  • NP_001070150()