NBR1 Antibody (5C3)

Western Blot: NBR1 Antibody (5C3) [H00004077-M05] - Analysis of NBR1 expression in transfected 293T cell line by NBR1 monoclonal antibody (M05A), clone 5C3. Lane 1: NBR1 transfected lysatE (107.4 KDa). Lane 2: Non-transfected lysate.

Western Blot: NBR1 Antibody (5C3) [H00004077-M05] - Western blot analysis of NBR1 over-expressed 293 cell line, cotransfected with NBR1 Validated Chimera RNAi or non-transfected control. Blot probed with H00004077-M05. GAPDH (36.1 kDa) used as loading control.

Sandwich ELISA: NBR1 Antibody (5C3) [H00004077-M05] - Detection limit for recombinant GST tagged NBR1 is approximately 0.03 ng/mL as a capture antibody.

Simple Western: NBR1 Antibody (5C3) [H00004077-M05] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Results from two hybridoma clones are shown. Simple Western image submitted by a verified customer review.

Product Discontinued

NBR1 Antibody (5C3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NBR1 (NP_005890, 2 a.a. - 96 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPV
• Specificity: NBR1 - neighbor of BRCA1 gene 1 (5C3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NBR1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • RNA Inhibition
  • Sandwich ELISA
  • Simple Western
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. This NBR1 Antibody (5C3) is validated for Simple Western from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NBR1 Antibody (5C3)

• Cell migration-inducing gene 19 protein
• FLJ98272
• KIAA0049CA125
• M17S2FLJ55359,1A1-3B
• Membrane component chromosome 17 surface marker 2,1A13B
• membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125)
• migration-inducing protein 19
• Neighbor of BRCA1 gene 1 protein
• neighbor of BRCA1 gene 1
• next to BRCA1 gene 1 protein
• Protein 1A1-3B

Background

The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many genes with transformation potential, but the function of this protein is unknown. This gene is located on a region of chromosome 17q21.1 that is in close proximity to tumor suppressor gene BRCA1. Three alternatively spliced variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NBR1 Antibody (H00004077-M05)(2)

Publications using H00004077-M05

• Kim HS, Kim Y, Lim MJ et al. The p38-activated ER stress-ATF6? axis mediates cellular senescence. FASEB J 2018-09-27 [PMID: 30260700]
• Yamashita S, Abe K, Tatemichi Y, Fujiki Y The membrane peroxin PEX3 induces peroxisome-ubiquitination-linked pexophagy. Autophagy 2014-09-01 [PMID: 25007327] (WB)

Review for NBR1 Antibody (H00004077-M05) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 12/05/2019

Summary

• Application: Simple Western
• Sample Tested: THP-1 macrophage lysate
• Species: Human
• Lot: I7261-5C3

Bioinformatics

• Gene Symbol: NBR1
• OMIM:
  • 166945(Human)