Nav1.7 Antibody

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Nav1.7 antibody (NBP3-48639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Nav1.7 antibody detects Nav1.7 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nav1.7 stained by Nav1.7 antibody (NBP3-48639) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nav1.7 antibody (NBP3-48639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Nav1.7 antibody detects Nav1.7 protein at cell membrane by immunofluorescent analysis.Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nav1.7 stained by Nav1.7 antibody (NBP3-48639) diluted at 1:500.Red: NF-H, an axon marker, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nav1.7 Antibody Summary

• Description: Novus Biologicals Rabbit Nav1.7 Antibody (NBP3-48639) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide encompassing a sequence within the Intracellular domain of mouse Nav1.7. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCN9A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Nav1.7 Antibody

• ETHA
• FEB3B
• hNE-Na
• Nav1.7
• NENA
• NE-NA
• NENAVoltage-gated sodium channel subunit alpha Nav1.7
• Neuroendocrine sodium channel
• Peripheral sodium channel 1
• PN1
• PN1hNE-Na
• SCN9A
• sodium channel protein type 9 subunit alpha
• Sodium channel protein type IX subunit alpha
• sodium channel, voltage-gated, type IX, alpha subunit
• voltage-gated sodium channel alpha subunit Nav1.7
• voltage-gated, type IX, alpha polypeptide

Background

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (from SwissProt).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCN9A