Napsin A Antibody (CL12954) - BSA Free

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Analysis in human kidney and skeletal muscle tissues using NBP3-18551 antibody. Corresponding NAPSA RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate to strong granular cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Staining of human lung cancer (adenocarcinoma) shows moderate to strong positivity in tumor cells.

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Napsin A Antibody (CL12954) [NBP3-18551] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu
• Applications: IHC
• Clone: CL12954
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Napsin A Antibody (CL12954) - BSA Free Summary

• Description: Novus Biologicals Mouse Napsin A Antibody (CL12954) - BSA Free (NBP3-18551) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from O96009, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPSA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Napsin A Antibody (CL12954) - BSA Free

• Asp 4
• ASP4
• Aspartyl protease 4
• EC 3.4.23
• EC 3.4.23.-
• EC 3.4.23.15
• EC 3.4.23.3
• EC 3.4.23.5
• KAP
• Kdap
• NAP1napsin-A
• NAPA
• NAPApronapsin A
• NAPSA
• napsin A aspartic peptidase
• Napsin A
• Napsin-1
• SNAPA
• TA01/TA02

Background

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPSA