Napsin A Antibody (CL12954) - Azide and BSA Free

Analysis in human kidney and skeletal muscle tissues using NBP3-44369 antibody. Corresponding Napsin A RNA-seq data are presented for the same tissues.

Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Staining of human cerebral cortex shows no positivity in neurons as expected.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human ovarian cancer (clear cell carcinoma) shows moderate to strong granular cytoplasmic positivity in tumor cells.

Staining of human lung cancer (adenocarcinoma) shows moderate to strong positivity in tumor cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL12954
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Napsin A Antibody (CL12954) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Napsin A Antibody (CL12954) - Azide and BSA Free (NBP3-18551) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of O96009, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPSA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Napsin A Antibody (CL12954) - Azide and BSA Free

• Asp 4
• ASP4
• Aspartyl protease 4
• EC 3.4.23
• EC 3.4.23.-
• EC 3.4.23.15
• EC 3.4.23.3
• EC 3.4.23.5
• KAP
• Kdap
• NAP1napsin-A
• NAPA
• NAPApronapsin A
• NAPSA
• napsin A aspartic peptidase
• Napsin A
• Napsin-1
• SNAPA
• TA01/TA02

Background

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPSA