NDFIP1 Antibody

Western blot shows lysates of A172 human glioblastoma cell line, SK-Mel-28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1-6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NDFIP1 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NDFIP1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NDFIP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human NDFIP1
  Ala10-Asp105
  Accession # Q9BT67
• Specificity: Detects human, mouse and rat NDFIP1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NDFIP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NDFIP1 Antibody

• Breast cancer-associated protein SGA-1M
• MGC10924
• N4WBP5
• N4WBP5Putative NF-kappa-B-activating protein 164
• NDFIP1
• Nedd4 family interacting protein 1
• NEDD4 family-interacting protein 1
• NEDD4 WW domain-binding protein 5
• Putative MAPK-activating protein PM13
• Putative NFKB and MAPK-activating protein

Background

NDFIP1 (NEDD4 family-interacting protein 1 and NEDD4 WW domain-binding protein 5) is a 26 kDa widely expressed protein. NDFIP1 is thought to be part of a family of integral Golgi membrane proteins. It serves as an adaptor for several E3 ubiquitin ligases including NEDD4 and ITCH, and consequently modulates the stability of their targets. NDFIP1 is genetically linked to human allergic and autoimmune disease, but the cellular mechanism by which these proteins enable foreign and self-antigens to be tolerated is unresolved. Over aa10-105, human NDFIP1 is 95% identical to mouse and rat NDFIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDFIP1
• Uniprot:
  • Q9BT67()