n-Myc [p Ser54] Antibody

Product Discontinued

n-Myc [p Ser54] Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region in the vicinity of Serine 54 when it is phosphorylated of mouse nMyc using the numbering given in entry Q810L7 (GeneID: 18109).
• Modification: p Ser54
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYCN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:200-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for n-Myc [p Ser54] Antibody

• BHLHE37
• bHLHe37N-myc proto-oncogene protein
• Class E basic helix-loop-helix protein 37
• MODED
• neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
• N-myc
• NMYCneuroblastoma MYC oncogene
• ODED
• oncogene NMYC
• pp65/67
• v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
• v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of 64 kDa and 67 kDa that exhibit relatively short (30 minute) half lives in vivo and exhibit DNA binding properties in vitro. Members of the Myc gene family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for n-Myc Antibody (NB100-79779)(2)

Publications using NB100-79779

• Wu J, Kharebava G, Piao C et al. Inhibition of E2F1/CDK1 pathway attenuates neuronal apoptosis in vitro and confers neuroprotection after spinal cord injury in vivo. PLoS One 2012-01-01 [PMID: 22848730]
• Sjostrom SK, Finn G, Hahn WC et al. The Cdk1 complex plays a prime role in regulating N-myc phosphorylation and turnover in neural precursors. Dev Cell 2005-09-01 [PMID: 16139224]

Bioinformatics

• Gene Symbol: MYCN
• Entrez:
  • 18109(Mouse)
• Uniprot:
  • Q810L7(Mouse)