N-Acetylmannosamine Kinase/GNE Antibody - Azide and BSA Free

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody [H00010020-D01P] - Analysis of GNE expression in transfected 293T cell line by GNE polyclonal antibody.Lane 1: GNE transfected lysate(79.30 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N-Acetylmannosamine Kinase/GNE Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit N-Acetylmannosamine Kinase/GNE Antibody - Azide and BSA Free (H00010020-D01P) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: GNE (NP_005467.1, 1 a.a. - 722 a.a.) full-length human protein. MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY
• Specificity: Reacts with glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N-Acetylmannosamine Kinase/GNE Antibody - Azide and BSA Free

• bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• DMRV
• GLCNE
• GLCNENM
• glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• GNE
• IBM2
• NAcetylmannosamine Kinase
• N-Acetylmannosamine Kinase
• N-acylmannosamine kinase
• Uae1
• UDP-GlcNAc-2-epimerase/ManAc kinase
• UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase

Background

The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNE
• Entrez:
  • 10020(Human)
• Uniprot:
  • NP_005467.1(Human)