N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody (JE64-81) [NBP3-32397] - Western blot analysis of N-Acetylmannosamine Kinase/GNE on different lysates with Rabbit anti-N-Acetylmannosamine Kinase/GNE antibody (NBP3-32397) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32397) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: JE64-81
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

N-Acetylmannosamine Kinase/GNE Antibody (JE64-81) Summary

• Description: Novus Biologicals Rabbit N-Acetylmannosamine Kinase/GNE Antibody (JE64-81) (NBP3-32397) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human N-Acetylmannosamine Kinase/GNE aa 673-722/722. (Uniprot: Q9Y223)
• Localization: Cytoplasm.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GNE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)

• bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• DMRV
• GLCNE
• GLCNENM
• glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• GNE
• IBM2
• NAcetylmannosamine Kinase
• N-Acetylmannosamine Kinase
• N-acylmannosamine kinase
• Uae1
• UDP-GlcNAc-2-epimerase/ManAc kinase
• UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase

Background

The protein encoded by the GNE gene is a bifunctional enzyme that monitors and begins biosynthesis of N-acetylneuraminic acid (NeuAc). NeuAC is a precursor of sialic acids and functions in early development as sialylation is a component of cell adhesion, signal transduction, and tumorigencity and metastic behavior of malignant cells. The GNE gene has been linked to various diseases such as: myopathy, dementia, glomerulosclerosis, muscular dystrophy, and neuromuscular disease. This protein creates an enzyme that is rate-limiting in the sialic acid biosynthetic pathway as well as plays a role in amino and nucleotide sugar metabolism as well as CMP-N-acetylneuraminate biosynthesis in eukaryotes. The GNE gene is known to interact with genes CRMP1. ZBTB16, PIK3C2A, KIAA1549, and RIF1 to participate in these pathways. GNE exists is five isoforms: isoform 1: 722 amino acids long; 79 kDA; isoform 2: 753 amino acids long, 83 kDA; isoform 3: 681 amino acids long; 74 kDA; isoform 4: 648 amino acids long, 71 kDA; isoform 5: 612 amino acids long, 66 kDA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNE