N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - Analysis of NAGK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NAGK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - Analysis of NAGK in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouseIgG Alexa fluor 488 conjugate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free Summary

• Description: Novus Biologicals Mouse N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free (NBP2-22554) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human N-Acetyl-D-Glucosamine Kinase/NAGK (1-344aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAGK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

• EC 2.7.1.59
• GlcNAc Kinase
• GNK
• GNKGlcNAc kinase
• N-acetyl-D-glucosamine kinase
• N-Acetylglucosamine Kinase
• N-acetylglucosamine kinaseHSA242910
• NAGK

Background

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P(II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAGK
• Uniprot:
  • NP_060037.2(Human)