Myosin light chain kinase Antibody (28C5) - BSA Free

Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate. All lanes: Myosin light chain kinase Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa Observed band size: 130-250 kDa

Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Overlay Peak curve showing A549 cells stained with Myosin light chain kinase Antibody (28C5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 28C5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myosin light chain kinase Antibody (28C5) - BSA Free Summary

• Description: Novus Biologicals Rabbit Myosin light chain kinase Antibody (28C5) - BSA Free (NBP3-26122) is a recombinant monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Myosin light chain kinase [UniProt Q15746]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYLK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Myosin light chain kinase Antibody (28C5) - BSA Free

• DKFZp686I10125
• EC 2.7.11
• EC 2.7.11.18
• FLJ12216
• Kinase-related protein
• KRP
• light polypeptide kinase
• MLCK1
• MLCK108
• MLCKMLCK210
• MSTP083
• MYLK1
• myosin light chain kinase
• smooth muscle myosin light chain kinase
• smooth muscle
• telokin

Background

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYLK