Myosin light chain 3 Antibody - BSA Free

Western Blot: Myosin light chain 3 Antibody - BSA Free [NBP3-47487] - HepG2 cells were subjected to SDS PAGE followed by western blot with (Myosin light chain 3 antibody) at dilution of 1:1000

Immunocytochemistry/ Immunofluorescence: Myosin light chain 3 Antibody - BSA Free [NBP3-47487] - Immunofluorescence analysis of U2OS cells using (Myosin light chain 3 antibody). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myosin light chain 3 Antibody - BSA Free Summary

• Immunogen: MYL3. (Uniprot# P08590)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Myosin light chain 3 Antibody - BSA Free

• Cardiac myosin light chain 1
• CMH8Ventricular/slow twitch myosin alkali light chain
• CMLC1
• light polypeptide 3, alkali; ventricular, skeletal, slow
• MLC1SBMyosin light chain 1, slow-twitch muscle B/ventricular isoform
• MLC1V
• myosin light chain 3
• myosin, light chain 3, alkali; ventricular, skeletal, slow
• VLC1

Background

Myosin light chain 3, or MYL3 for short, consists of a 195 amino acid isoform that is 22 kDa, and is involved in the regulation of Myosin, which is a protein that conducts ATP hydrolysis. Current research is being conducted on the relationship between Myosin light chain 3 and a multitude of diseases and disorders, including familial hypertrophic cardiomyopathy, congestive heart failure, restrictive cardiomyopathy, dilated cardiomyopathy, diabetes mellitus, and renal failure. Myosin light chain 3 has been linked to the RhoA pathway, as well as PKA signaling, growth cone motility, cell adhesion, cardiac muscle contraction, and cytoskeleton remodeling. The protein interacts with YWHAQ, MYH13, YWHAZ, MYH9, and MYH7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYL3