Myosin Light Chain 2 Antibody (1F7) - BSA Free

Immunohistochemistry: Myosin Light Chain 2 Antibody (1F7) [NBP3-43002] - Immunohistochemistry of paraffin-embedded human heart slide using (Myosin Light Chain 2 Antibody) at dilution of 1:50

Western Blot: Myosin Light Chain 2 Antibody (1F7) [NBP3-43002] - human heart tissue were subjected to SDS PAGE followed by western blot with (Myosin Light Chain 2 antibody) at dilution of 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Myosin Light Chain 2 Antibody (1F7) - BSA Free Summary

• Description: Novus Biologicals Mouse Myosin Light Chain 2 Antibody (1F7) - BSA Free (NBP3-43002) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: myosin, light chain 2, regulatory, cardiac, slow - (Uniprot# P10916)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYL2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Myosin Light Chain 2 Antibody (1F7) - BSA Free

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYL2