MyoD Antibody (5.2F)

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Formalin fixed paraffin embedded human rhabdomyosarcoma stained with MyoD antibody (NB120-788).

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Staining of rhabdomyosarcoma.

• Reactivity: Hu, Mu
• Applications: IHC
• Clone: 5.2F
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MyoD Antibody (5.2F) Summary

• Description: Novus Biologicals Mouse MyoD Antibody (5.2F) (NB120-788) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length Mouse MyoD1 protein.
• Epitope: This antibody is specific to an epitope of amino acid 3-56 in mouse MyoD1.
• Localization: Nuclear
• Specificity: This antibody is specific to a 45 kD protein, which is indenitified as MyoD1. It does not react with myogenin, Myf5 or Myf6. This antibody stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for MyoD Antibody (5.2F)

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Background

MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MyoD Antibody (NB120-788). (Showing 1 - 2 of 2 FAQ).

1. For the following product, could you let us know the buffer composition and the concentration of sodium azide?
   • Our lab has confirmed that NB120-788 is supplied in PBS with 2% BSA and 0.05% sodium azide.
2. What is the concentration of this product?
   • The concentration of the antibody for lot A418 is 97.56 ug/ml

Bioinformatics

• Gene Symbol: MYOD1
• Entrez:
  • 17927(Mouse)