MYF-5 Antibody (593128) [Alexa Fluor® 488]

Images

 
C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF‑5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4027G, filled histogram) or isotype control antibody (Catalog # IC003G, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
593128
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

MYF-5 Antibody (593128) [Alexa Fluor® 488] Summary

Immunogen
E. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349
Specificity
Detects human MYF-5 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MYF5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYF-5 Antibody (593128) [Alexa Fluor® 488]

  • bHLHc2
  • bHLHc2Class C basic helix-loop-helix protein 2
  • MYF5
  • MYF-5
  • myogenic factor 5

Background

MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for MYF-5 Antibody (IC4027G) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYF5
Uniprot