MyBPC3 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

MyBPC3 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse MyBPC3
  Arg998-Lys1100
  Accession # O70468
• Specificity: Detects human and mouse MyBPC3 in Western blots and in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MyBPC3 Antibody [Alexa Fluor® 700]

• Cardiac MyBP-C
• CMH4
• C-protein, cardiac muscle isoform
• DKFZp779E1762
• FHC
• MYBP-C
• MyBPC3
• myosin binding protein C, cardiac
• myosin-binding protein C, cardiac
• myosin-binding protein C, cardiac-type

Background

MyBPC3 (Myosin-binding protein C-cardiac type) is a 140-150 kDa member of the MyBP family, Ig superfamily of proteins. It is expressed in cardiac muscle, and contributes both to myosin filament structure by interacting with light meromysin, and the regulation of contraction by binding to myosin subfragment-2, which results in a reduction of actomyosin ATPase activity. Mouse MyBPC3 is 1270 amino acids (aa) in length. It contains five consecutive C2-type Ig-like domains (aa 151-767), three FN type III repeats (aa 768-958), and two additional C-terminal Ig-like domains (aa 967-1270). There are at least three utilized phosphorylation sites and one Pro‑rich region (aa 100-150). In human, multiple mutations generate variable-length premature truncated forms of MyBPC3. There are two potential isoform variants. One basically shows a six aa substitution for aa 339-344, while a second contains the same six aa substitution coupled to an eight aa extension at the N-terminus. Over aa 998‑1100, mouse MyBPC3 shares 89% and 95% aa identity with human and rat MyBPC3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.