Mxi1 Antibody

Immunocytochemistry/Immunofluorescence: Mxi1 Antibody [NBP2-97478] - Immunofluorescence staining of Mxi1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Mxi1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP2-97478] - Immunochemical staining of human Mxi1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP2-97478] - Immunochemical staining of human Mxi1 in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Discontinued

Mxi1 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Mxi1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MXI1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Mxi1 Antibody

• BHLHC11
• Class C basic helix-loop-helix protein 11
• MAD2
• MAX dimerization protein 2
• MAX interacting protein 1
• MAX interactor 1bHLHc11MAD2
• max-interacting protein 1
• Max-related transcription factor
• MGC43220
• MXD2
• MXI
• Mxi1
• MXI-WR

Background

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MXI1