MVP Antibody

Knockout Validated: MVP Antibody [NBP1-33560] - Wild-type (WT) and MVP/LRP knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MVP/LRP antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MVP Antibody [NBP1-33560] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE MVP/LRP antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MVP Antibody [NBP1-33560] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: MVP Antibody [NBP1-33560] - Mouse colon. MVP/LRP antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MVP Antibody [NBP1-33560] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: MVP Antibody [NBP1-33560] - A549 xenograft, using LRP(MVP) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: MVP Antibody [NBP1-33560] - Human breast carcinoma. MVP/LRP antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Ca, Fe
• Applications: WB, ICC/IF, IHC, IHC-P, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MVP Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MVP/LRP. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus, nuclear pore complex
• Predicted Species: Rat (90%), Canine (91%), Feline (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MVP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (93%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MVP Antibody

• LRPVAULT1
• Lung resistance-related protein
• major vault protein

Background

This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for MVP Antibody (NBP1-33560). (Showing 1 - 1 of 1 FAQ).

1. Are there any MVP antibodies that will recognize mouse and can be used for immunofluorescence?
   • At this time we do not have an MVP antibody that will recognize mouse and has been used for immunofluorescence. If you would like to try an antibody in an untested species or application you would qualify for our Innovator's Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Gene Symbol: MVP