MVK Overexpression Lysate (Native)

Images

 
Western Blot: MVK Overexpression Lysate (Adult Normal) [NBL1-13401] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MVK.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MVK Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201971 and based on accession number NM_000431. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mevalonate kinase (MVK), transcript variant 1, mRNA.
Gene
MVK

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MVK

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MVK Overexpression Lysate (Native)

  • EC 2.7.1.36
  • LH receptor mRNA-binding protein
  • LRBP
  • mevalonate kinase (mevalonic aciduria)
  • mevalonate kinase 1
  • mevalonate kinase
  • Mevalonic Aciduria
  • MKFLJ96772
  • MVK
  • MVLK
  • POROK3

Background

This gene encodes the peroxisomal enzyme mevalonate kinase. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive, hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Two transcript variants that encode the same protein have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MVK Lysate (NBL1-13401) (0)

There are no publications for MVK Lysate (NBL1-13401).
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Video Protocols

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Bioinformatics

Gene Symbol MVK
Entrez