Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence)

ELISA: Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence) [NBP2-66710] - Samples were spiked with high concentrations of Rat Muscle Phosphofructokinase/PFKM/PFK-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence) [NBP2-66710] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL

Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Rat 6-PFK in samples. No significant cross-reactivity or interference between Rat 6-PFK and analogues was observed.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 10.9%
• Intra-Assay: CV% < 9.25%
• Spike Recovery: 88-112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: PFKM

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Muscle Phosphofructokinase/PFKM/PFK-1 ELISA Kit (Chemiluminescence)

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PFKM