Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NLS1334] - Analysis of anti-CHRM5 antibody with human brain, neurons and glia at 10 ug/ml.

Product Discontinued

Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CHRM5.
• Specificity: Human CHRM5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (94%), Monkey (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRM5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:52 - 1:250
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23212463).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (100%), Marmoset (100%), Gorilla (94%), Orangutan (94%), Gibbon (94%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

• cholinergic receptor, muscarinic 5
• CHRM5
• HM5
• M5
• M5R
• mAChR M5
• MGC41838
• muscarinic acetylcholine receptor M5

Background

Muscarinic cholinergic receptor M5 is an Acetylcholine Receptor that is active during embryonic development. It is coupled to phosphoinositide degradation and to the activation of mitogen-activated protein kinase (MAPK). The M5 receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (NLS1334)(2)

Publications using NLS1334

• Tangeman L, McIlhatton MA, Grierson P et al. Regulation of BLM Nucleolar Localization. Genes (Basel) 2016-09-21 [PMID: 27657136]
• Fong G, Backman LJ, Andersson G et al. Human tenocytes are stimulated to proliferate by acetylcholine through an EGFR signalling pathway Cell Tissue Res 2012-12-05 [PMID: 23212463] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CHRM5
• Entrez:
  • 1133(Human)
• Uniprot:
  • P08912()