Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (590123) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human CHRM5 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human CHRM5 Monoclonal Antibody (Catalog # MAB10323) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 590123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (590123) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CHRM5
  Met1-Pro532
  Accession # P08912
• Specificity: Detects human CHRM5 in diect ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (590123) [Unconjugated]

• cholinergic receptor, muscarinic 5
• CHRM5
• HM5
• M5
• M5R
• mAChR M5
• MGC41838
• muscarinic acetylcholine receptor M5

Background

Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Stimulation of CHRM5 is known to increase cyclic AMP levels. The clinical implications of CHRM5 are unknown but it may be linked to schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08912()