Musashi-2 Antibody - BSA Free

Western Blot: Musashi-2 Antibody [NBP1-76575] - Analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 5 ug/mL.

Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody - BSA Free [NBP1-76575] - Immunofluorescence of Musashi-2 in mouse brain tissue with Musashi-2 Antibody at 20 ug/mL.Green: Musashi-2 antibody Red: Phylloidin staining Blue: DAPI staining

Immunohistochemistry: Musashi-2 Antibody - BSA Free [NBP1-76575] - Immunohistochemistry of Musashi-2 in mouse brain tissue with Musashi-2 Antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Musashi-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Musashi-2 Antibody - BSA Free (NBP1-76575) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MSI2. The immunogen is located within amino acids 30 - 80 of MSI2. Amino Acid Squence: MVMRDPTTKRSRGF
• Specificity: MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSI2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  Musashi-2 Peptide (NBP1-76575PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Musashi-2 Antibody - BSA Free

• FLJ36569
• MGC3245
• MSI2
• MSI2H
• musashi homolog 2 (Drosophila)
• Musashi2
• Musashi-2
• RNA-binding protein Musashi homolog 2

Background

Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSI2
• Uniprot:
  • EAW94506(Human)