Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody [NBP1-31207] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MURF1 protein stained by MURF1 antibody [N1], N-term diluted at 1:200. ...read more
Immunohistochemistry-Frozen: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse diaphragm, 20X; MURF1 and DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NBP1-31207] - Paraffin-embedded Breast ca, using antibody at 1:500 dilution.
40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4 using NBP1-31207 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Immunogen affinity purified
Alternate Names for MuRF1/TRIM63 Antibody
Iris RING finger protein
muscle specific ring finger protein 2
Muscle-specific RING finger protein 1
RING finger protein 28FLJ32380
RNF28E3 ubiquitin-protein ligase TRIM63
Striated muscle RING zinc finger protein
tripartite motif containing 63
tripartite motif-containing 63
Tripartite motif-containing protein 63
This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse skeletal muscle proteins were separated by 4-12% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:500 in 1% non-fat milk.
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