MuRF1/TRIM63 Antibody

Images

 
Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody [NBP1-31207] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MURF1 protein stained by MURF1 antibody [N1], N-term diluted at 1:200. ...read more
Immunohistochemistry-Frozen: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse diaphragm, 20X; MURF1 and DAPI. Image from verified customer review.
Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NBP1-31207] - Paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MuRF1/TRIM63 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MURF1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM63
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-Fr usage reported by customer review.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-31207 in the following applications:

Reactivity Notes

Mouse reactivity reported by customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MuRF1/TRIM63 Antibody

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for MuRF1/TRIM63 Antibody (NBP1-31207) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: WB, IHC-Fr.

Reviews using NBP1-31207:
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WB
(1)
IHC-Fr
(1)
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(2)
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Images Ratings Applications Species Date Details
Western Blot MuRF1/TRIM63 NBP1-31207
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reviewed by:
Francesca Pacifici
WB Mouse 09/26/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
Lot9D113535

Comments

CommentsWestern Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse skeletal muscle proteins were separated by 4-12% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:500 in 1% non-fat milk.
Immunohistochemistry-Frozen MuRF1/TRIM63 NBP1-31207
Enlarge
reviewed by:
Jake VanBelzen
IHC-Fr Mouse 09/24/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Diaphragm
SpeciesMouse

Blocking

Blocking Details5% goat normal serum-PBS; 15min @ RT

Primary Anitbody

Dilution Ratio1:50 in PBS; 60min at 37 C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-FITC
Secondary Manufacturer Cat#F9887
Secondary Concentration1:300

Details

Detection NotesFluorescent microscope; 20x
Fixation DetailsFrozen in OCT; Sectioned at 8uM; PBS-1% Triton 20min @ RT; Wash with PBS 3x 5min, RT
Wash DescriptionPBS 3x 5min each

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MuRF1/TRIM63 Antibody (NBP1-31207) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for MuRF1/TRIM63 Antibody (NBP1-31207)

Discover related pathways, diseases and genes to MuRF1/TRIM63 Antibody (NBP1-31207). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Francesca Pacifici
09/26/2017
Application: WB
Species: Mouse

Jake VanBelzen
09/24/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol TRIM63
Entrez