MuRF1/TRIM63 Antibody

Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP1-31207] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MURF1 protein stained by MURF1 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Frozen: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse diaphragm, 20X; MURF1 and DAPI. Image from verified customer review.

Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse skeletal muscle proteins were separated by 4-12% SDS-PAGE. This image was submitted via customer Review.

Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - MURF1 antibody detects MURF1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:1000.

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NBP1-31207] - Human breast cancer, using MURF1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Discontinued

MuRF1/TRIM63 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MURF1. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM63
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: MuRF1/TRIM63 antibody validated for IHC-F from a verified customer review.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MuRF1/TRIM63 Antibody

• EC 6.3.2.-
• IRF
• IRFMURF2
• Iris RING finger protein
• MuRF1
• MuRF-1
• muscle specific ring finger protein 2
• Muscle-specific RING finger protein 1
• RING finger protein 28FLJ32380
• RNF28
• RNF28E3 ubiquitin-protein ligase TRIM63
• SMRZ
• SMRZMURF-1
• Striated muscle RING zinc finger protein
• TRIM63
• tripartite motif containing 63
• tripartite motif-containing 63
• Tripartite motif-containing protein 63

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MuRF1/TRIM63 Antibody (NBP1-31207)(2)

Publications using NBP1-31207

• Pacifici F, Della-Morte D, Piermarini F et al. Prdx6 Plays a Main Role in the Crosstalk Between Aging and Metabolic Sarcopenia Antioxidants (Basel) 2020-04-17 [PMID: 32316601] (WB, Mouse)
• Lim JA, Sun B, Puertollano R, Raben N Therapeutic Benefit of Autophagy Modulation in Pompe Disease Mol Ther. 2018-07-04 [PMID: 29804932] (WB, Mouse)

Reviews for MuRF1/TRIM63 Antibody (NBP1-31207) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Francesca Pacifici WB Mouse 09/26/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle homogenate
• Species: Mouse
• Lot: 9D113535

Comments

• Comments: Western Blot: MuRF1/TRIM63 Antibody [NBP1-31207] - Mouse skeletal muscle proteins were separated by 4-12% SDS-PAGE, and the membrane was blotted with MURF1 antibody diluted by 1:500 in 1% non-fat milk.

reviewed by: Jake VanBelzen IHC-Fr Mouse 09/24/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Diaphragm
• Species: Mouse

Bioinformatics

• Gene Symbol: TRIM63