MUC1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

MUC1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli‑derived recombinant human MUC-1
  Pro126-Arg145
  Accession # P15941
• Specificity: Detects human MUC‑1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MUC1 Antibody [Alexa Fluor® 647]

• Breast carcinoma-associated antigen DF3
• Carcinoma-associated mucin
• CD227 antigen
• CD227
• DF3 antigen
• EMA
• Episialin
• H23 antigen
• H23AG
• KL-6
• MAM6
• MUC-1
• MUC1/ZD
• mucin 1, cell surface associated
• mucin 1, transmembrane
• Mucin-1
• Peanut-reactive urinary mucin
• PEM
• PEMMUC-1/SEC
• PEMT
• Polymorphic epithelial mucin
• PUMMUC-1/X
• tumor associated epithelial mucin
• Tumor-associated epithelial membrane antigen
• Tumor-associated mucin

Background

MUC-1 (Mucin-1; also episialin, MAM-6, PEM and PUM) is a 150-450 kDa type I transmembrane glycoprotein that belongs to the Mucin family of molecules. It forms a protective barrier against microbes on the apical surface of most glandular epithelium, and becomes prominently displayed on tumor cells. Mature human MUC-1 is 1232 amino acids (aa) in length. It contains an 1135 aa extracellular domain (ECD) (aa 24-1158), and a signal transducing 73 aa cytoplasmic tail (aa 1182-1255). The ECD is highly polymorphic as evidenced by a highly variable number of 20 aa O-glycosylated tandem repeats. Intracellular processing cleaves MUC-1 between Gly1097 and Ser1098, generating a noncovalently linked heterodimer that is expressed on the cell surface. The C-terminus is 22-25 kDa in size, while the N-terminus is highly variable (70-400 kDa in size), a function of the number of resident tandem repeats. Cell surface processing by TACE releases a soluble ECD fragment of variable MW. There are multiple splice variants that show massive reductions in the size of the ECD. Over aa 126-145, human MUC-1 shares < 50% aa identity with mouse MUC-1. When compared to human, mouse MUC-1 is found not to be polymorphic and possess poorly conserved tandem repeat sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MUC1 Antibody (AF6298R). (Showing 1 - 1 of 1 FAQ).

1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
   • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Bioinformatics

• Uniprot:
  • P15941()