MUC1 Antibody (HMPV) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
HMPV
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

MUC1 Antibody (HMPV) [Janelia Fluor® 646] Summary

Immunogen
Human breast cancer cell line ZR-75 cells
Localization
Cytoplasmic and cell surface
Marker
Epithelial Marker
Specificity
This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Alternate Names for MUC1 Antibody (HMPV) [Janelia Fluor® 646]

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for MUC1 Antibody (NBP2-47883JF646). (Showing 1 - 1 of 1 FAQ).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47883AF350
Alexa Fluor 405 NBP2-47883AF405
Alexa Fluor 488 NBP2-47883AF488
Alexa Fluor 532 NBP2-47883AF532
Alexa Fluor 594 NBP2-47883AF594
Alexa Fluor 647 NBP2-47883AF647
Alexa Fluor 700 NBP2-47883AF700
Alexa Fluor 750 NBP2-47883AF750
Allophycocyanin NBP2-47883APC
Allophycocyanin/Cy7 NBP2-47883APCCY7
Biotin NBP2-47883B
DyLight 350 NBP2-47883UV
DyLight 405 NBP2-47883V
DyLight 488 NBP2-47883G
DyLight 550 NBP2-47883R
DyLight 594 NBP2-47883DL594
DyLight 650 NBP2-47883C
DyLight 680 NBP2-47883FR
DyLight 755 NBP2-47883IR
FITC NBP2-47883F
HRP NBP2-47883H
Janelia Fluor 549 NBP2-47883JF549
Janelia Fluor 646 NBP2-47883JF646
PE NBP2-47883PE
PE/Atto594 NBP2-47883PEATT594
PE/Cy5.5 NBP2-47883PECY55
PE/Cy7 NBP2-47883PECY7
PerCP NBP2-47883PCP

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Bioinformatics

Gene Symbol MUC1
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