MUC1 Antibody (2336A) [Unconjugated]

MCF-7 human breast cancer cell line was stained with Rabbit Anti-Human MUC-1 Monoclonal Antibody (Catalog # MAB62981, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0110). Staining was performed using our Staining Membrane-associated Proteins protocol.

MUC‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human MUC‑1 Monoclonal Antibody (Catalog # MAB62981) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clone: 2336A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MUC1 Antibody (2336A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human MUC-1
  Met1-Ser380
  Accession # P15941
• Specificity: Detects human MUC-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MUC1 Antibody (2336A) [Unconjugated]

• Breast carcinoma-associated antigen DF3
• Carcinoma-associated mucin
• CD227 antigen
• CD227
• DF3 antigen
• EMA
• Episialin
• H23 antigen
• H23AG
• KL-6
• MAM6
• MUC-1
• MUC1/ZD
• mucin 1, cell surface associated
• mucin 1, transmembrane
• Mucin-1
• Peanut-reactive urinary mucin
• PEM
• PEMMUC-1/SEC
• PEMT
• Polymorphic epithelial mucin
• PUMMUC-1/X
• tumor associated epithelial mucin
• Tumor-associated epithelial membrane antigen
• Tumor-associated mucin

Background

MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MUC1 Antibody (MAB62981). (Showing 1 - 1 of 1 FAQ).

1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
   • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Bioinformatics

• Uniprot:
  • P15941()