mtTFA Antibody

Western Blot: mtTFA Antibody [NBP2-29657] - rat kidney lysate showing specific immunolabeling of the ~24k TFAM protein.

Product Discontinued

mtTFA Antibody Summary

• Immunogen: Native recombinant mouse TFAM protein with c-terminal 6-his tag. Accession # P40630.
• Specificity: Specific for the ~24 kDa TFAM protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFAM
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody has been directly tested for reactivity in human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for mtTFA Antibody

• mitochondrial transcription factor A
• MtTF1
• mtTFA
• TCF-6
• TCF6L1
• TCF6L2Mitochondrial transcription factor 1
• TCF6TCF6L3
• Transcription factor 6
• Transcription factor 6-like 2 (mitochondrial transcription factor)
• Transcription factor 6-like 2
• transcription factor A, mitochondrial

Background

Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFAM
• Entrez:
  • 21780(Mouse)
• Uniprot:
  • P40630(Mouse)