MTHFD2 Antibody (4G7-2G3) - Azide and BSA Free

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Analysis of MTHFD2 expression in HepG2.

Immunohistochemistry-Paraffin: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - Analysis of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 3 ug/ml.

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - Analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.Lane 1: MTHFD2 transfected lysate(26.8 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4G7-2G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MTHFD2 Antibody (4G7-2G3) - Azide and BSA Free Summary

• Immunogen: MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN
• Localization: Mitochondrial
• Specificity: MTHFD2 - methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTHFD2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MTHFD2 Antibody (4G7-2G3) - Azide and BSA Free

• bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
• methenyltetrahydrofolate cyclohydrolase
• methylene tetrahydrofolate dehydrogenase (NAD+ dependent)
• methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2
• mitochondrial
• NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
• NMDMC

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTHFD2 Antibody (H00010797-M01)(4)

Publications using H00010797-M01

• Wang Y, Hongu T, Nishimura T et al. Mitochondrial one-carbon metabolic enzyme MTHFD2 facilitates mammary gland development during pregnancy Biochemical and Biophysical Research Communications 2023-06-01 [PMID: 37450958] (IHC-P, Human)
  
  Details:
  1:100 dilution
• Sha Y, Luogen P, Omar E et al. One carbon metabolism in human lung cancer. Transl Lung Cancer Res. 2021-06-15 [PMID: 34295659]
• Gustafsson Sheppard N, Jarl L, Mahadessian D et al. The folate-coupled enzyme MTHFD2 is a nuclear protein and promotes cell proliferation. Sci Rep 2015-01-01 [PMID: 26461067] (IF/IHC)
• Nilsson Roland, Jain Mohit, Madhusudhan Nikhil et al. Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer. Nat Commun. 2014-01-01 [PMID: 24451681] (IF/IHC)

Bioinformatics

• Gene Symbol: MTHFD2
• Entrez:
  • 10797(Human)
• OMIM:
  • 604887(Human)
• Uniprot:
  • AAH01548(Human)