MTA3 Antibody

Western Blot: MTA3 Antibody [NB100-2306] - Nuclear extract (25 mcg for WB; 0.5 mg for IP) from MCF7 cells. Affinity purified rabbit anti-MTA3 antibody NB100-2306 used at 1 mcg/ml for WB and 5 mcg/0.5 mg extract for IP.

Immunohistochemistry-Paraffin: MTA3 Antibody [NB100-2306] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MTA3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Discontinued

MTA3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human metastasis associated 3 using the numbering given in entry Swiss-Prot entry Q9BTC8 (GeneID 57504)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:500-1:5000
• Application Notes: In WB, detects band at ~60 kDa. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  MTA3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTA3 Antibody

• member 3
• metastasis associated 1 family, member 3
• metastasis-associated protein MTA3

Background

MTA3 plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. MTA3 gene encodes for two forms, a 515 amino acids and a longer protein of 594 amino acids, called MTA3L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTA3 Antibody (NB100-2306)(5)

Publications using NB100-2306

• Pakala SB, Bui-Nguyen TM, Reddy SD et al. Regulation of NF-kappaB circuitry by a component of the nucleosome remodeling and deacetylase complex controls inflammatory response homeostasis. J Biol Chem 2010-07-01 [PMID: 20519513]
• Li DQ, Ohshiro K, Khan MN et al. Requirement of MTA1 in ATR-mediated DNA damage checkpoint function. J Biol Chem 2010-06-01 [PMID: 20427275]
• Li M, Aliotta JM, Asara JM et al. Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture. J Biol Chem 2010-02-01 [PMID: 20026604]
• Li DQ, Ohshiro K, Reddy SD et al. E3 ubiquitin ligase COP1 regulates the stability and functions of MTA1. Proc Natl Acad Sci U S A 2009-10-01 [PMID: 19805145]
• Zhang H, Stephens LC, Kumar R et al. Metastasis tumor antigen family proteins during breast cancer progression and metastasis in a reliable mouse model for human breast cancer. Clin Cancer Res 2006-03-01 [PMID: 16533771]

Bioinformatics

• Gene Symbol: MTA3
• Entrez:
  • 57504(Human)
• Uniprot:
  • Q9BTC8(Human)