MST2/STK3 Antibody (3C3) - BSA Free

Western Blot: MST2/STK3 Antibody (3C3) [NBP3-26298] - Positive Western Blot detected in: U251 whole cell lysate, U87 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate. All lanes: MST2/STK3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 60 kDa Observed band size: 50-70 kDa

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MST2/STK3 Antibody (3C3) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human MST2/STK3 [UniProt Q13188]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STK3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MST2/STK3 Antibody (3C3) - BSA Free

• EC 2.7.11
• FLJ90748
• KRS1
• Mammalian STE20-like protein kinase 2
• Mess1
• MST2
• MST-2
• MST2EC 2.7.11.1
• MST3
• serine/threonine kinase 3 (STE20 homolog, yeast)
• serine/threonine kinase 3 (Ste20, yeast homolog)
• serine/threonine kinase 3
• serine/threonine-protein kinase 3
• Serine/threonine-protein kinase Krs-1
• STE20-like kinase MST2
• STK3

Background

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STK3