MRTO4 Antibody - Azide and BSA Free

Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa

Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).

Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRTO4 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human MRTO4 (Uniprot#: Q9UKD2; Met1-Asp239)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRTO4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRTO4 Antibody - Azide and BSA Free

• C1orf33
• chromosome 1 open reading frame 33
• dJ657E11.4
• mRNA turnover 4 homolog (S. cerevisiae)
• mRNA turnover protein 4 homolog
• MRT4, mRNA turnover 4, homolog (S. cerevisiae)
• MRT4, mRNA turnover 4, homolog
• MRT460S acidic ribosomal protein PO

Background

C1orf33 encodes a protein sharing a low level of sequence similarity with ribosomal protein P0. While the precise function of the encoded protein is currently unknown, it appears to be involved in mRNA turnover and ribosome assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRTO4