MRPL52 Antibody

Western Blot: MRPL52 Antibody [NBP1-86094] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-354

Immunocytochemistry/Immunofluorescence: MRPL52 Antibody [NBP1-86094] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.

Immunohistochemistry-Paraffin: MRPL52 Antibody [NBP1-86094] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Western Blot: MRPL52 Antibody [NBP1-86094] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MRPL52 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QWRLQQGLAANPSGYGPLTELPDWSYADGRPAPPMKGQLRRKAERETFARRVVLLSQEMDAGLQAWQLRQQKLQEEQRKQENALKPKGASLKSPLPSQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL52
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MRPL52 Protein (NBP1-86094PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MRPL52 Antibody

• L52mt
• mitochondrial ribosomal protein L52
• MRP-L52,39S ribosomal protein L52, mitochondrial

Background

MRPL52, or Mitochondrial Ribosomal Protein L52, contains three different isoforms that are 14 kDa, 7 kDa, and 13 kDa, and is involved in protein synthesis within the 39S large mitochondrial ribosome subunit. Current research is being conducted on the relationship between MRPL52 and a variety of genetic diseases. MRPL52 is not known to interact with any other proteins, but is linked to the biological process of translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL52