MRP3 Overexpression Lysate (Native)

Images

 
Western Blot: MRP3 Overexpression Lysate (Adult Normal) [NBL1-07178] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRP3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MRP3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221522 and based on accession number NM_003786. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens ATP-binding cassette, sub-family C (CFTR/MRP), member 3 (ABCC3), transcript variant 1, mRNA.
Gene
ABCC3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
169.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MRP3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MRP3 Overexpression Lysate (Native)

  • ABC31
  • ATP-binding cassette sub-family C member 3
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 3
  • canalicular multispecific organic anion transporter 2
  • canicular multispecific organic anion transporter
  • cMOAT2
  • EC 3.6.3
  • EC 3.6.3.44
  • EC 6.3.2.4
  • MLP2DKFZp686E22157
  • MRP3MOAT-DEST90757
  • multidrug resistance associated protein
  • Multidrug resistance-associated protein 3
  • Multi-specific organic anion transporter D

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MRP3 Lysate (NBL1-07178) (0)

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Video Protocols

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Bioinformatics

Gene Symbol ABCC3