MRP1 Antibody (MRPm6)

Immunohistochemistry: MRP1 Antibody (MRPm6) [NBP1-42340]

Product Discontinued

MRP1 Antibody (MRPm6) Summary

• Immunogen: Raised against a bacterial fusion protein of MRP, containing a segment of 170 amino acids in the carboxy terminal end and part of the carboxy proximal nucleotide binding domain of the protein
• Specificity: Reacts with an internal epitope of MRP1. Does not cross-react with the human MDR1 and MDR3 gene products
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20-1:50
  • Immunohistochemistry 1:20-50
  • Immunohistochemistry-Frozen 1:20-50
  • Immunohistochemistry-Paraffin 1:20-50
  • Western Blot 1:20-1:50
• Application Notes: MRPm6 has potential value for detection of MRP MDR in human tumor samples. Working dilutions. Flow cytometry: at least 1:20-1:50. Western blotting: at least 1:20-1:50. Immunocytochemistry: aceton fixed cell preparation at least 1:20-50. Immunohistochemistry: For use of paraffin embedded sections a 0.01M citrate pretreatment (10 min 100 degrees C) may increase the performance of this antibody. In Flow cytometry fixate cells in 10% (v/v) Lysing solution followed by primairy antibody and anti-Mouse-FITC.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Serum free tissue culture supernatant, 0.7% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MRP1 Antibody (MRPm6)

• ABC29
• ABCC
• ABCC1
• ATP-binding cassette sub-family C member 1
• ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• EC 3.6.3
• EC 3.6.3.44
• GS-X
• Leukotriene C(4) transporter
• LTC4 transporter
• MRP1
• MRP1DKFZp781G125
• MRPDKFZp686N04233
• multidrug resistance associated protein 1
• multidrug resistance protein
• multidrug resistance-associated protein 1
• multiple drug resistance protein 1
• multiple drug resistance-associated protein

Background

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCC1