MRP1 Antibody (MRPm5)

Product Discontinued

MRP1 Antibody (MRPm5) Summary

• Immunogen: The antibody reacts with the MRP1 antigen.
• Specificity: MRPm5 reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPm5 was raised against a bacterial fusion protein of MRP1, containing amino acids 986-1204 of the protein. MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: MRPm5 has potential value for detection of MRP MDR in human tumor samples. Immunocytochemistry: use 1:20-50 dil. on acetone or paraformaldehyde (4% in PBS) fixed cytospin preparations. For immunohistochemistry: MRPm5 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG (1:25) and a monoclonal mouse APAAP complex (1:50). MRPm5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumours, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 min. Flow cytometry: use 1:20-50 dil. after fixing cells in 10% Lysing solution in H2O, and anti-mouse-FITC. Western blotting: use 1:20-50 dil, and anti-mouse-HRP.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.1% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for MRP1 Antibody (MRPm5)

• ABC29
• ABCC
• ABCC1
• ATP-binding cassette sub-family C member 1
• ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• EC 3.6.3
• EC 3.6.3.44
• GS-X
• Leukotriene C(4) transporter
• LTC4 transporter
• MRP1
• MRP1DKFZp781G125
• MRPDKFZp686N04233
• multidrug resistance associated protein 1
• multidrug resistance protein
• multidrug resistance-associated protein 1
• multiple drug resistance protein 1
• multiple drug resistance-associated protein

Background

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRP1 Antibody (NBP1-42341)(1)

Publication using NBP1-42341

• Kurihara Y, Sawazumi T, Takeuchi T. Exploration of interactions between membrane proteins embedded in supported lipid bilayers and their antibodies by reflectometric interference spectroscopy-based sensing. Analyst. 2014-10-15 [PMID: 25270526] (Human)
  
  Details:
  MRP1 antibody used for detection of Human MRP1 vesicles via microfluidic reflectometric interference spectroscopy (RIfS)-based sensor.

Bioinformatics

• Gene Symbol: ABCC1