MRGPRF Antibody (916219) [Unconjugated]

Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MRGPRF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with either (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 916219
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MRGPRF Antibody (916219) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human MRGPRF
  Met1-Ser343
  Accession # NP_659452
• Specificity: Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRGPRF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MRGPRF Antibody (916219) [Unconjugated]

• FLJ16111
• FLJ29034
• FLJ40998
• FLJ53714
• G protein-coupled receptor 140
• G protein-coupled receptor 168
• G protein-coupled receptor MrgF
• GPR140
• GPR168
• G-protein coupled receptor 140
• G-protein coupled receptor 168
• mas-related G protein-coupled MRGF
• Mas-related gene F protein
• MAS-related GPR, member F
• mas-related G-protein coupled receptor member F
• MGC21621
• mrgF
• MRGFDKFZp586B2122
• MRGPRF
• RTA
• seven transmembrane helix receptor

Background

MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).

1. Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRGPRF Antibody (MAB8396)(1)

Publication using MAB8396

• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288]

Bioinformatics

• Gene Symbol: MRGPRF
• Uniprot:
  • NP_659452()