Mouse TIMP-1 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse TIMP-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TIMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse TIMP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TIMP-1 showed linea...r curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse TIMP-1.
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• Specificity: Natural and recombinant mouse TIMP-1. This kit detects approximately 80% of the recombinant mouse TIMP-1 complexed with the pro-form of recombinant mouse MMP-9 and approximately 60% when complexed with active recombinant mouse MMP-9.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Timp1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse TIMP-1 Quantikine ELISA Kit

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIMP-1 (MTM100)(29)

Publications using MTM100

• Sugawara, T;Hitaka, K;Matsumoto, M;Nakamura, S;Kobayashi, R;Kandori, H;Nio, Y; Differences between therapeutic mechanisms of resmetirom and semaglutide against MASH in western diet-fed MC4R-knockout mice Scientific reports 2025-11-20 [PMID: 41266634] (Mouse)
• Braunreiter, K;Kempton, A;Mejia-Guerra, MK;Murray, A;Baine, S;Adegboye, K;Haile, A;Kumar Ahuja, SJ;Fedoce, A;Liu, C;Burch, P;Kabadi, AM; Characterization of a humanized mouse model of Duchenne muscular dystrophy to support the development of genetic medicines Disease models & mechanisms 2025-10-01 [PMID: 41104521] (Mouse)
• Yoshida, Y;Nishikawa, N;Fukuoka, K;Tsuruta, A;Otsuki, K;Fukuda, T;Terada, Y;Tanihara, T;Kumamoto, T;Tsukamoto, R;Nishi, T;Oyama, K;Hamamura, K;Mayanagi, K;Koyanagi, S;Ohdo, S;Matsunaga, N; Monocyte/Macrophage-Specific Loss of ARNTL Suppresses Chronic Kidney Disease-Associated Cardiac Impairment International journal of molecular sciences 2024-12-03 [PMID: 39684718] (Transgenic Mouse)
• Kadam, AH;Schnitzer, JE; Highly Calibrated Relationship Between Bleomycin Concentrations and Facets of the Active Phase Fibrosis in Classical Mouse Bleomycin Model International journal of molecular sciences 2024-11-15 [PMID: 39596365] (Mouse)
• Yulyaningsih, E;Suh, JH;Fanok, M;Chau, R;Solanoy, H;Takahashi, R;Bakardjiev, AI;Becerra, I;Benitez, NB;Chiu, CL;Davis, SS;Dowdle, WE;Earr, T;Estrada, AA;Gill, A;Ha, C;Haddick, PCG;Henne, KR;Larhammar, M;Leung, AW;Maciuca, R;Memarzadeh, B;Nguyen, HN;Nugent, AA;Osipov, M;Ran, Y;Rebadulla, K;Roche, E;Sandman, T;Wang, J;Lewcock, JW;Scearce-Levie, K;Kane, LA;Sanchez, PE; DNL343 is an investigational CNS penetrant eukaryotic initiation factor 2B activator that prevents and reverses the effects of neurodegeneration caused by the integrated stress response eLife 2024-09-17 [PMID: 39287504] (Mouse)
• Nakamura, K;Tsukasaki, M;Tsunematsu, T;Yan, M;Ando, Y;Huynh, NC;Hashimoto, K;Gou, Q;Muro, R;Itabashi, A;Iguchi, T;Okamoto, K;Nakamura, T;Nakano, K;Okamura, T;Ueno, T;Ito, K;Ishimaru, N;Hoshi, K;Takayanagi, H; The periosteum provides a stromal defence against cancer invasion into the bone Nature 2024-08-21 [PMID: 39169177] (Mouse, Transgenic Mouse)
• Jin, Q;Zhang, C;Chen, R;Jiang, L;Li, H;Wu, P;Li, L; Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic Journal of translational medicine 2024-04-15 [PMID: 38622667] (Transgenic Mouse)
• Z Wang, J Hazen, X Jia, E Org, Y Zhao, LJ Osborn, N Nimer, J Buffa, MK Culley, D Krajcik, BH van den Bo, K Zwinderman, BS Levison, M Nieuwdorp, AJ Lusis, JA DiDonato, SL Hazen The Nutritional Supplement L-Alpha Glycerylphosphorylcholine Promotes Atherosclerosis International Journal of Molecular Sciences, 2021-12-15;22(24):. 2021-12-15 [PMID: 34948275] (Mouse)
• H Ge, G Liu, TM Yamawaki, C Tao, ST Alexander, K Ly, P Fordstrom, AA Shkumatov, CM Li, S Rajamani, M Zhou, B Ason Phytosterol accumulation results in ventricular arrhythmia, impaired cardiac function and death in mice Scientific Reports, 2021-08-31;11(1):17449. 2021-08-31 [PMID: 34465831] (Mouse, Transgenic Mouse)
• HH Hansen, HM Ægidius, D Oró, SS Evers, S Heebøll, PL Eriksen, KL Thomsen, A Bengtsson, SS Veidal, M Feigh, MP Suppli, FK Knop, H Grønbæk, D Miranda, JL Trevaskis, N Vrang, J Jelsing, KTG Rigbolt Human translatability of the GAN diet-induced obese mouse model of non-alcoholic steatohepatitis BMC Gastroenterol, 2020-07-06;20(1):210. 2020-07-06 [PMID: 32631250] (Mouse)
• G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier Int J Mol Sci, 2020-05-28;21(11):. 2020-05-28 [PMID: 32481745] (Mouse)
• M Matsumoto, H Yashiro, H Ogino, K Aoyama, T Nambu, S Nakamura, M Nishida, X Wang, DM Erion, M Kaneko Acetyl-CoA carboxylase 1 and 2 inhibition ameliorates steatosis and hepatic fibrosis in a MC4R knockout murine model of nonalcoholic steatohepatitis PLoS ONE, 2020-01-28;15(1):e0228212. 2020-01-28 [PMID: 31990961] (Mouse)
• CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Mouse)
• Z D'Costa, K Jones, A Azad, R van Stipho, SY Lim, AL Gomes, P Kinchesh, SC Smart, WG McKenna, FM Buffa, OJ Sansom, RJ Muschel, E O'Neill, E Fokas Gemcitabine-induced TIMP1 attenuates therapy response and promotes tumor growth and liver metastasis in pancreatic cancer Cancer Res., 2017-08-01;0(0):. 2017-08-01 [PMID: 28765154] (Mouse)
• Monocyte chemotactic protein-1 deficiency reduces spontaneous metastasis of Lewis lung carcinoma in mice fed a high-fat diet Oncotarget, 2016-04-26;7(17):24792-9. 2016-04-26 [PMID: 27028862] (Mouse)
• Yan L, DeMars L Effects of a high-fat diet on spontaneous metastasis of Lewis lung carcinoma in plasminogen activator inhibitor-1 deficient and wild-type mice. PLoS ONE, 2014-10-30;9(10):e110869. 2014-10-30 [PMID: 25356654] (Mouse)
• Besnard A, Struyf S, Guabiraba R, Fauconnier L, Rouxel N, Proost P, Uyttenhove C, Van Snick J, Couillin I, Ryffel B CXCL6 antibody neutralization prevents lung inflammation and fibrosis in mice in the bleomycin model. J Leukoc Biol, 2013-08-23;94(6):1317-23. 2013-08-23 [PMID: 23975892] (Mouse)
• Hassan F, Ren D, Zhang W Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS). PLoS ONE, 2012-05-24;7(5):e37912. 2012-05-24 [PMID: 22655080] (Mouse)
• van Putten M, Hulsker M, Nadarajah VD, van Heiningen SH, van Huizen E, van Iterson M, Admiraal P, Messemaker T, den Dunnen JT, 't Hoen PA, Aartsma-Rus A The effects of low levels of dystrophin on mouse muscle function and pathology. PLoS ONE, 2012-02-16;7(2):e31937. 2012-02-16 [PMID: 22359642] (Mouse)
• Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence. J Neuroinflammation, 2011-09-09;8(0):115. 2011-09-09 [PMID: 21906275] (Mouse)
• Meissburger B, Stachorski L, Roder E, Rudofsky G, Wolfrum C Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans. Diabetologia, 2011-03-25;54(6):1468-79. 2011-03-25 [PMID: 21437772] (Mouse)
• Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. PLoS ONE, 2009-11-19;4(11):e7916. 2009-11-19 [PMID: 19936259] (Mouse)
• Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. FASEB J., 2009-08-31;24(1):70-80. 2009-08-31 [PMID: 19720619] (Mouse)
• Lackey DE, Ashley SL, Davis AL, Hoag KA Retinoic acid decreases adherence of murine myeloid dendritic cells and increases production of matrix metalloproteinase-9. J. Nutr., 2008-08-01;138(8):1512-9. 2008-08-01 [PMID: 18641199] (Mouse)
• Morschl E, Molina JG, Volmer JB, Mohsenin A, Pero RS, Hong JS, Kheradmand F, Lee JJ, Blackburn MR A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis. Am. J. Respir. Cell Mol. Biol., 2008-06-27;39(6):697-705. 2008-06-27 [PMID: 18587054] (Mouse)
• Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1. Am. J. Pathol., 2007-11-30;171(6):1762-73. 2007-11-30 [PMID: 18055551] (Mouse)
• Madan M, Bishayi B, Hoge M, Amar S Atheroprotective role of interleukin-6 in diet- and/or pathogen-associated atherosclerosis using an ApoE heterozygote murine model. Atherosclerosis, 2007-04-06;197(2):504-14. 2007-04-06 [PMID: 17412346] (Mouse)
• Davidsen ML, Wurtz SØ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis. Br. J. Cancer, 2006-10-23;95(8):1114-20. 2006-10-23 [PMID: 17047657] (Mouse)
• Hu QW, Liu GT Effects of bicyclol on dimethylnitrosamine-induced liver fibrosis in mice and its mechanism of action. Life Sci., 2006-02-28;79(6):606-12. 2006-02-28 [PMID: 16603200] (Mouse)

FAQs for TIMP-1 (MTM100). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Timp1