Mouse SP-D Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse SP-D Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse SP-D immunoassay is a 4.5 hour solid-phase ELISA designed to measure SP-D in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse SP-D and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SP-D. Results obtained using natural mouse ...SP-D showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SP-D.
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• Specificity: Natural and recombinant mouse SP-D.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Sftpd

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse SP-D Quantikine ELISA Kit

• COLEC7collectin-7
• Collectin 7
• Collectin-7
• Lung surfactant protein D
• PSPD
• PSP-D
• SFTP4
• SFTP4pulmonary surfactant-associated protein D
• SFTPD
• SPD
• SP-D
• SP-Dpulmonary surfactant apoprotein
• surfactant protein D
• surfactant, pulmonary-associated protein D
• surfactant-associated protein, pulmonary 4

Background

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for SP-D (MSFPD0)(5)

Publications using MSFPD0

• Petersen, AG;Korntner, SH;Bousamaki, J;Oró, D;Arraut, AM;Pors, SE;Salinas, CG;Andersen, MW;Madsen, MR;Nie, Y;Butts, J;Roqueta-Rivera, M;Simonsen, U;Hansen, HH;Feigh, M; Reproducible lung protective effects of a TGF?R1/ALK5 inhibitor in a bleomycin-induced and spirometry-confirmed model of IPF in male mice Physiological reports 2024-10-01 [PMID: 39394052] (Mouse)
• J Zhao, N Zhen, Q Zhou, J Lou, W Cui, G Zhang, B Tian NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982193] (Mouse)
• IM Imtiazul, R Asma, JH Lee, NJ Cho, S Park, HY Song, HW Gil Change of surfactant protein D and A after renal ischemia reperfusion injury PLoS ONE, 2019-12-26;14(12):e0227097. 2019-12-26 [PMID: 31877195] (Mouse)
• CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein Macrophage migration inhibitory factor enhances influenza-associated mortality in mice JCI Insight, 2019-07-11;4(13):. 2019-07-11 [PMID: 31292300] (Mouse)
• WE Eddy, KQ Gong, B Bell, WC Parks, SF Ziegler, AM Manicone Stat5 Is Required for CD103(+) Dendritic Cell and Alveolar Macrophage Development and Protection from Lung Injury J. Immunol., 2017-05-12;0(0):. 2017-05-12 [PMID: 28500076] (Mouse)

FAQs for SP-D (MSFPD0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Sftpd