Mouse G-CSF Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse G-CSF Quantikine ELISA Kit Summary

Background
The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed ...dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.
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Specificity
Natural and recombinant mouse G-CSF
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Csf3

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MCS00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse G-CSF Quantikine ELISA Kit

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • Pluripoietin

Background

Mouse granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation and activation of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa) precursor protein with a 30 aa signal sequence that is proteolytically cleaved to form a 178 aa O-glycosylated mature protein containing two intrachain disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form and a minor alternatively spliced 207 aa form of G-CSF precursors, have been isolated. Mouse and human G-CSF are 76% identical at the aa sequence level and the two proteins show species cross-reactivity. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IL-1. Other cell types, including fibroblasts, endothelial cells, astrocytes and bone marrow stroma cells, can also secrete G-CSF after activation. In addition, various tumor cells express G-CSF constitutively.  

Mouse G-CSF receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs to the hematopoietin receptor superfamily. The mature protein is 812 amino acids in length and contains a 601 aa extracellular region, a 24 aa transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular region contains multiple modules, including an N-terminal Ig-like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Based on crystallographic study, G-CSF receptor forms a complex with the ligand in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity. Cells known to express G-CSF R include monocytes and neutrophils, megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic progenitors, trophoblasts, endothelial cells and various tumor cell types.  

G-CSF is an important regulator for granulopoiesis in vivo. It has been demonstrated that G-CSF can support the growth of multi-lineage hematopoietic progenitor cells without influencing their commitment to the myeloid lineage and mobilize hematopoietic progenitor cells from the bone marrow into the bloodstream. On mature neutrophils, G-CSF may regulate neutrophil survival by controlling their rate of apoptosis. G-CSF has also been shown to enhance the functional capacity of mature neutrophils. As a consequence of its effects on hematopoietic progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the presence M-CSF. Within the peripheral blood stem cell population mobilized with G-CSF, selective increases in the number of T helper 2-inducing dendritic cells are found.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for G-CSF (MCS00)(41)

We have publications tested in 1 confirmed species: Mouse.


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Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using MCS00 Applications Species
M Bosiljcic, RA Cederberg, MJ Hamilton, NE LePard, BT Harbourne, JL Collier, EC Halvorsen, R Shi, SE Franks, AY Kim, JP Banáth, M Hamer, FM Rossi, KL Bennewith Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs Breast Cancer Res., 2019;21(1):103. 2019 [PMID: 31488209] (Mouse) Mouse
Z Li, J Hardij, SS Evers, CR Hutch, SM Choi, Y Shao, BS Learman, KT Lewis, RL Schill, H Mori, DP Bagchi, SM Romanelli, KS Kim, E Bowers, C Griffin, RJ Seeley, K Singer, DA Sandoval, CJ Rosen, OA MacDougald G-CSF partially mediates effects of sleeve gastrectomy on the bone marrow niche J. Clin. Invest., 2019;129(6):2404-2416. 2019 [PMID: 31063988] (Mouse) Mouse
FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2 JCI Insight, 2019;4(9):. 2019 [PMID: 31045582] (Mouse) Mouse
JM Ubellacker, N Baryawno, N Severe, MJ DeCristo, J Sceneay, JN Hutchinson, MT Haider, CS Rhee, Y Qin, WM Gregory, AC Garrido-Ca, I Holen, JE Brown, RE Coleman, DT Scadden, SS McAllister Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer Cancer Res., 2018;0(0):. 2018 [PMID: 30065048] (Mouse) Mouse
M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29695634] (Mouse) Mouse
N Guo, V DeAngelis, C Zhu, EH Schuchman, CM Simonaro Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice JIMD Rep, 2018;0(0):. 2018 [PMID: 29654542] (Mouse) Mouse
MC Okondo, SD Rao, CY Taabazuing, AJ Chui, SE Poplawski, DC Johnson, DA Bachovchin Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome Cell Chem Biol, 2018;0(0):. 2018 [PMID: 29396289] (Mouse) Mouse
CJ Kurkjian, H Guo, ND Montgomery, N Cheng, H Yuan, JR Merrill, GD Sempowski, WJ Brickey, JP Ting The Toll-Like Receptor 2/6 Agonist, FSL-1 Lipopeptide, Therapeutically Mitigates Acute Radiation Syndrome Sci Rep, 2017;7(1):17355. 2017 [PMID: 29230065] (Mouse) Mouse
TS Kapellos, C Recio, DR Greaves, AJ Iqbal Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia Mediators Inflamm., 2017;2017(0):4315412. 2017 [PMID: 28852269] (Mouse) Mouse
W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling Sci Rep, 2017;7(1):8402. 2017 [PMID: 28827748] (Mouse) Mouse
Show All 41 Publications.

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FAQs for G-CSF (MCS00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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Bioinformatics

Gene Symbol Csf3