Mouse G-CSF Quantikine ELISA Kit Summary
The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed ...dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.
Natural and recombinant mouse G-CSF
Solid Phase Sandwich ELISA
No significant interference observed with available related molecules.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse G-CSF Quantikine ELISA Kit
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
- granulocyte colony-stimulating factor
granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric
glycoprotein that regulates the proliferation, differentiation and activation
of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa)
precursor protein with a 30 aa signal sequence that is proteolytically cleaved
to form a 178 aa O-glycosylated mature protein containing two intrachain
disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form
and a minor alternatively spliced 207 aa form of G-CSF precursors, have been
isolated. Mouse and human G-CSF are 76% identical at the aa sequence level and
the two proteins show species cross-reactivity. G-CSF is produced primarily by
monocytes and macrophages upon activation by endotoxin, TNF-alpha or IL-1. Other
cell types, including fibroblasts, endothelial cells, astrocytes and bone
marrow stroma cells, can also secrete G-CSF after activation. In addition,
various tumor cells express G-CSF constitutively.
receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs
to the hematopoietin receptor superfamily. The mature protein is 812 amino
acids in length and contains a 601 aa extracellular region, a 24 aa
transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular
region contains multiple modules, including an N-terminal Ig-like domain, a
cytokine receptor homology domain, and three fibronectin type III domains.
Based on crystallographic study, G-CSF receptor forms a complex with the ligand
in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity.
Cells known to express G-CSF R include monocytes and neutrophils,
megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic
progenitors, trophoblasts, endothelial cells and various tumor cell types.
G-CSF is an
important regulator for granulopoiesis in vivo. It has been demonstrated that
G-CSF can support the growth of multi-lineage hematopoietic progenitor cells
without influencing their commitment to the myeloid lineage and mobilize
hematopoietic progenitor cells from the bone marrow into the bloodstream. On
mature neutrophils, G-CSF may regulate neutrophil survival by controlling their
rate of apoptosis. G-CSF has also been shown to enhance the functional capacity
of mature neutrophils. As a consequence of its effects on hematopoietic
progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the
presence M-CSF. Within the peripheral blood stem cell population mobilized with
G-CSF, selective increases in the number of T helper 2-inducing dendritic cells
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for G-CSF (MCS00). (Showing 1 - 1 of 1 FAQs).
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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