Mouse G-CSF DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse G-CSF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Csf3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse G-CSF DuoSet ELISA, 15 Plate

• C17orf33
• chromosome 17 open reading frame 33
• colony stimulating factor 3 (granulocyte)
• CSF3
• CSF3OS
• Filgrastim
• GCSF
• G-CSF
• GCSFlenograstim
• granulocyte colony-stimulating factor
• Lenograstim
• MGC45931
• Pluripoietin

Background

Mouse granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation and activation of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa) precursor protein with a 30 aa signal sequence that is proteolytically cleaved to form a 178 aa O-glycosylated mature protein containing two intrachain disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form and a minor alternatively spliced 207 aa form of G-CSF precursors, have been isolated. Mouse and human G-CSF are 76% identical at the aa sequence level and the two proteins show species cross-reactivity. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IL-1. Other cell types, including fibroblasts, endothelial cells, astrocytes and bone marrow stroma cells, can also secrete G-CSF after activation. In addition, various tumor cells express G-CSF constitutively.   

Mouse G-CSF receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs to the hematopoietin receptor superfamily. The mature protein is 812 amino acids in length and contains a 601 aa extracellular region, a 24 aa transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular region contains multiple modules, including an N-terminal Ig-like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Based on crystallographic study, G-CSF receptor forms a complex with the ligand in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity. Cells known to express G-CSF R include monocytes and neutrophils, megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic progenitors, trophoblasts, endothelial cells and various tumor cell types.    

G-CSF is an important regulator for granulopoiesis in vivo. It has been demonstrated that G-CSF can support the growth of multi-lineage hematopoietic progenitor cells without influencing their commitment to the myeloid lineage and mobilize hematopoietic progenitor cells from the bone marrow into the bloodstream. On mature neutrophils, G-CSF may regulate neutrophil survival by controlling their rate of apoptosis. G-CSF has also been shown to enhance the functional capacity of mature neutrophils. As a consequence of its effects on hematopoietic progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the presence M-CSF. Within the peripheral blood stem cell population mobilized with G-CSF, selective increases in the number of T helper 2-inducing dendritic cells are found.

Publications for G-CSF (DY414)(32)

Publications using DY414

• Zeydabadinejad, S;Kim, JSA;Zheng, A;Kandalgaonkar, MR;Ababio, PB;Vijay-Kumar, M;Yeoh, BS;Saha, P; Targeting anemia-induced CD71+ reticulocytes protects mice from Plasmodium infection Infection and Immunity 2025-07-01 [PMID: 40590713] (Mouse)
• Hameed, M;Hossain, MS;Daamen, AR;Lipsky, PE;Weger-Lucarelli, J; Granulocyte colony-stimulating factor protects against arthritogenic alphavirus pathogenesis in a type I IFN-dependent manner bioRxiv : the preprint server for biology 2024-10-13 [PMID: 39416071] (Mouse, Transgenic Mouse)
• de Oliveira, LD;de Carvalho, LS;Xavier, ACC;de Oliveira, FE;Leão, MVP;Diamantino, MGG;Khoury, RD;Valera, MC;Carvalho, CAT;Abu Hasna, A; In Vitro Evaluation of Sodium Hypochlorite, Chlorhexidine, Propolis, and Calcium Hydroxide Effect on Lipoteichoic-Acid-Induced Proinflammatory Cytokines Production Dentistry journal 2024-09-05 [PMID: 39329852] (Mouse)
• Flores-Valdez, MA;Peterson, EJR;Aceves-Sánchez, MJ;Baliga, NS;Morita, YS;Sparks, IL;Saini, DK;Yadav, R;Lang, R;Mata-Espinosa, D;León-Contreras, JC;Hernández-Pando, R; Comparison of the transcriptome, lipidome, and c-di-GMP production between BCG?BCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages Scientific reports 2024-05-24 [PMID: 38789479] (Mouse, Transgenic Mouse)
• Kinsella, RL;Kimmey, JM;Smirnov, A;Woodson, R;Gaggioli, MR;Chavez, SM;Kreamalmeyer, D;Stallings, CL; Autophagy prevents early proinflammatory responses and neutrophil recruitment during Mycobacterium tuberculosis infection without affecting pathogen burden in macrophages PLoS biology 2023-06-15 [PMID: 37319285] (Mouse)
• N Ikeda, H Kubota, R Suzuki, M Morita, A Yoshimura, Y Osada, K Kishida, D Kitamura, A Iwata, S Yotsumoto, D Kurotaki, K Nishimura, A Nishiyama, T Tamura, T Kamatani, T Tsunoda, M Murakawa, Y Asahina, Y Hayashi, H Harada, Y Harada, A Yokota, H Hirai, T Seki, M Kuwahara, M Yamashita, S Shichino, M Tanaka, K Asano The early neutrophil-committed progenitors aberrantly differentiate into immunoregulatory monocytes during emergency myelopoiesis Cell Reports, 2023-02-28;42(3):112165. 2023-02-28 [PMID: 36862552] (Mouse)
• P Selvam, CM Cheng, HU Dahms, VK Ponnusamy, YY Sun AhR Mediated Activation of Pro-Inflammatory Response of RAW 264.7 Cells Modulate the Epithelial-Mesenchymal Transition Toxics, 2022-10-27;10(11):. 2022-10-27 [PMID: 36355934] (Mouse)
• M Kokoszynsk, ND Ubags, JJ Bivona, S Ventrone, LF Reed, AE Dixon, MJ Wargo, ME Poynter, BT Suratt Storage conditions of high-fat diets affect pulmonary inflammation Physiological Reports, 2021-11-01;9(22):e15116. 2021-11-01 [PMID: 34822216] (Mouse)
• JH Song, DY Eum, SY Park, YH Jin, JW Shim, SJ Park, MY Kim, SJ Park, K Heo, YJ Choi Inhibitory effect of ginsenoside Rg3 on cancer stemness and mesenchymal transition in breast cancer via regulation of myeloid-derived suppressor cells PLoS ONE, 2020-10-22;15(10):e0240533. 2020-10-22 [PMID: 33091036] (Mouse)
• AL Aldrich, CE Heim, W Shi, RW Fallet, B Duan, T Kielian TLR2 and caspase-1 signaling are critical for bacterial containment but not clearance during craniotomy-associated biofilm infection J Neuroinflammation, 2020-04-14;17(1):114. 2020-04-14 [PMID: 32290861] (Mouse)
• NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 2019-12-09;0(0):. 2019-12-09 [PMID: 31819009] (Mouse)
• S Lorscheid, A Müller, J Löffler, C Resch, P Bucher, FC Kurschus, A Waisman, K Schäkel, S Hailfinger, K Schulze-Os, D Kramer Keratinocyte-derived I&kappaB? drives psoriasis and associated systemic inflammation JCI Insight, 2019-11-14;4(22):. 2019-11-14 [PMID: 31622280] (Mouse)
• BE Hsu, S Tabariès, RM Johnson, S Andrzejews, J Senecal, C Lehuédé, MG Annis, EH Ma, S Völs, L Ramsay, R Froment, A Monast, IR Watson, Z Granot, RG Jones, J St-Pierre, PM Siegel Immature Low-Density Neutrophils Exhibit Metabolic Flexibility that Facilitates Breast Cancer Liver Metastasis Cell Rep, 2019-06-25;27(13):3902-3915.e6. 2019-06-25 [PMID: 31242422] (Mouse)
• MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Nat Commun, 2018-03-28;9(1):1250. 2018-03-28 [PMID: 29593283] (Mouse)
• I Mitroulis, K Ruppova, B Wang, LS Chen, M Grzybek, T Grinenko, A Eugster, M Troullinak, A Palladini, I Kourtzelis, A Chatzigeor, A Schlitzer, M Beyer, LAB Joosten, B Isermann, M Lesche, A Petzold, K Simons, I Henry, A Dahl, JL Schultze, B Wielockx, N Zamboni, P Mirtschink, Ü Coskun, G Hajishenga, MG Netea, T Chavakis Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity Cell, 2018-01-11;172(1):147-161.e12. 2018-01-11 [PMID: 29328910] (Mouse)
• MS Gresnigt, M Jaeger, RK Subbarao M, O Rasid, G Jouvion, C Fitting, WJG Melchers, TD Kanneganti, A Carvalho, O Ibrahim-Gr, FL van de Vee The Absence of NOD1 Enhances Killing of Aspergillus fumigatus Through Modulation of Dectin-1 Expression Front Immunol, 2017-12-13;8(0):1777. 2017-12-13 [PMID: 29326692] (Mouse)
• I Mitroulis, LS Chen, RP Singh, I Kourtzelis, M Economopou, T Kajikawa, M Troullinak, A Ziogas, K Ruppova, K Hosur, T Maekawa, B Wang, P Subramania, T Tonn, P Verginis, M von Bonin, M Wobus, M Bornhäuser, T Grinenko, M Di Scala, A Hidalgo, B Wielockx, G Hajishenga, T Chavakis Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche J. Clin. Invest., 2017-08-28;0(0):. 2017-08-28 [PMID: 28846069] (Mouse)
• Extracellular Superoxide Dismutase Enhances the Recruitment of Immature Neutrophils to the Liver Infect Immun, 2016-11-18;0(0):. 2016-11-18 [PMID: 27600509] (Mouse)
• Epidermal RAF prevents allergic skin disease Elife, 2016-07-19;5(0):. 2016-07-19 [PMID: 27431613] (Mouse)
• The time-of-day of myocardial infarction onset affects healing through oscillations in cardiac neutrophil recruitment EMBO Mol Med, 2016-08-01;0(0):. 2016-08-01 [PMID: 27226028] (Mouse)
• Reducing hypoxia and inflammation during invasive pulmonary aspergillosis by targeting the Interleukin-1 receptor Sci Rep, 2016-05-24;6(0):26490. 2016-05-24 [PMID: 27215684] (Mouse)
• A Savers, O Rasid, M Parlato, M Brock, G Jouvion, B Ryffel, JM Cavaillon, G Eberl, O Ibrahim-Gr Infection-Mediated Priming of Phagocytes Protects against Lethal Secondary Aspergillus fumigatus Challenge PLoS ONE, 2016-04-14;11(4):e0153829. 2016-04-14 [PMID: 27078879] (Mouse)
• Zhao Y, Olonisakin T, Xiong Z, Hulver M, Sayeed S, Yu M, Gregory A, Kochman E, Chen B, Mallampalli R, Sun M, Silverstein R, Stolz D, Shapiro S, Ray A, Ray P, Lee J Thrombospondin-1 restrains neutrophil granule serine protease function and regulates the innate immune response during Klebsiella pneumoniae infection. Mucosal Immunol, 2014-12-10;8(4):896-905. 2014-12-10 [PMID: 25492474] (Mouse)
• Cao Y, Slaney C, Bidwell B, Parker B, Johnstone C, Rautela J, Eckhardt B, Anderson R BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity. Cancer Res, 2014-09-15;74(18):5091-102. 2014-09-15 [PMID: 25224959] (Mouse)
• De Filippo K, Neill D, Mathies M, Bangert M, McNeill E, Kadioglu A, Hogg N A new protective role for S100A9 in regulation of neutrophil recruitment during invasive pneumococcal pneumonia. FASEB J, 2014-04-28;28(8):3600-8. 2014-04-28 [PMID: 24776746] (Mouse)
• Weber S, Wetzel S, Prox J, Lehmann T, Schneppenheim J, Donners M, Saftig P Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10). Biochem Biophys Res Commun, 2013-11-15;442(3):234-41. 2013-11-15 [PMID: 24239882] (Mouse)
• Bosmann M, Haggadone M, Zetoune F, Sarma J, Ward P The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation. Eur J Immunol, 2013-05-13;43(7):1907-13. 2013-05-13 [PMID: 23575697] (Mouse)
• von Vietinghoff S, Asagiri M, Azar D Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice. J. Immunol., 2010-06-02;185(1):670-8. 2010-06-02 [PMID: 20519647] (Mouse)
• von Vietinghoff S, Ley K IL-17A controls IL-17F production and maintains blood neutrophil counts in mice. J. Immunol., 2009-06-19;183(2):865-73. 2009-06-19 [PMID: 19542376] (Mouse)
• Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice. J. Immunol., 2007-12-15;179(12):8274-9. 2007-12-15 [PMID: 18056371] (Mouse)
• Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int., 2007-01-17;71(6):514-25. 2007-01-17 [PMID: 17228364] (Mouse)
• Allen GB, Suratt BT, Rinaldi L, Petty JM, Bates JH Choosing the frequency of deep inflation in mice: balancing recruitment against ventilator-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-12;291(4):L710-7. 2006-05-12 [PMID: 16698851] (Mouse)

FAQs for G-CSF (DY414). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Csf3