Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse Fas Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fas Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Fas Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Fas Lig...and{\f9\i showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Fas Ligand.
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• Specificity: Natural and recombinant mouse Fas Ligand
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FASLG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit

• apoptosis (APO-1) antigen ligand 1
• Apoptosis antigen ligand
• APT1LG1CD95L
• APTL
• CD178 antigen
• CD178
• CD95L
• CD95-L
• Fas antigen ligand
• Fas ligand (TNF superfamily, member 6)
• Fas Ligand
• FASLCD95 ligand
• FASLG
• TNFSF6
• TNFSF6FasL
• tumor necrosis factor (ligand) superfamily, member 6
• tumor necrosis factor ligand superfamily member 6

Background

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Fas Ligand/TNFSF6 (MFL00)(13)

Publications using MFL00

• Lung Injury Combined with Loss of Regulatory T Cells Leads to De Novo Lung-Restricted Autoimmunity J Immunol, 2016-05-18;0(0):. 2016-05-18 [PMID: 27194786] (Mouse)
• de Claro RA, Zhu X, Tang J, Morgan-Stevenson V, Schwartz BR, Iwata A, Liles WC, Raines EW, Harlan JM Hematopoietic Fas deficiency does not affect experimental atherosclerotic lesion formation despite inducing a proatherogenic state. Am. J. Pathol., 2011-05-06;178(6):2931-7. 2011-05-06 [PMID: 21550016] (Mouse)
• Leu SW, Shi L, Xu C, Zhao Y, Liu B, Li Y, Shiedlin A, Xiang C, Shen H, Quinn DA, Hales CA, Zhao H TLR4 through IFN-beta promotes low molecular mass hyaluronan-induced neutrophil apoptosis. J. Immunol., 2010-11-22;186(1):556-62. 2010-11-22 [PMID: 21098223] (Mouse)
• Murthy A, Defamie V, Smookler DS Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice. J. Clin. Invest., 2010-07-12;120(8):2731-44. 2010-07-12 [PMID: 20628198] (Mouse)
• Beck JM, Preston AM, Wilcoxen SE, Morris SB, Sturrock A, Paine R Critical roles of inflammation and apoptosis in improved survival in a model of hyperoxia-induced acute lung injury in Pneumocystis murina-infected mice. Infect. Immun., 2009-01-05;77(3):1053-60. 2009-01-05 [PMID: 19124601] (Mouse)
• Bem RA, van Woensel JB, Bos AP, Koski A, Farnand AW, Domachowske JB, Rosenberg HF, Martin TR, Matute-Bello G Mechanical ventilation enhances lung inflammation and caspase activity in a model of mouse pneumovirus infection. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-07;296(1):L46-56. 2008-11-07 [PMID: 18996903] (Mouse)
• Kierstein S, Krytska K, Sharma S, Amrani Y, Salmon M, Panettieri RA, Zangrilli J, Haczku A Ozone inhalation induces exacerbation of eosinophilic airway inflammation and hyperresponsiveness in allergen-sensitized mice. Allergy, 2008-04-01;63(4):438-46. 2008-04-01 [PMID: 18315731] (Mouse)
• Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia. Cell Death Differ., 2007-10-26;15(1):143-51. 2007-10-26 [PMID: 17962815] (Mouse)
• Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis. J. Immunol., 2007-03-15;178(6):3379-89. 2007-03-15 [PMID: 17339432] (Mouse)
• Niu J, Azfer A, Deucher MF, Goldschmidt-Clermont PJ, Kolattukudy PE Targeted cardiac expression of soluble Fas prevents the development of heart failure in mice with cardiac-specific expression of MCP-1. J. Mol. Cell. Cardiol., 2006-05-05;40(6):810-20. 2006-05-05 [PMID: 16678847] (Mouse)
• Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts. Circulation, 2004-09-14;110(11):II194-9. 2004-09-14 [PMID: 15364862] (Mouse)
• Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease. Blood, 2004-07-27;104(12):3789-96. 2004-07-27 [PMID: 15280201] (Mouse)
• Hao Z, Hampel B, Yagita H, Rajewsky K T cell-specific ablation of Fas leads to Fas ligand-mediated lymphocyte depletion and inflammatory pulmonary fibrosis. J. Exp. Med., 2004-05-17;199(10):1355-65. 2004-05-17 [PMID: 15148335] (Mouse)

FAQs for Fas Ligand/TNFSF6 (MFL00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FASLG