Mouse CCL4/MIP-1 beta DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CCL4/MIP-1 beta DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL4/MIP-1 beta DuoSet ELISA, 15 Plate

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.

Publications for CCL4/MIP-1 beta (DY451)(21)

Publications using DY451

• Watanabe, T;Hayashi, M;Arai, M;Matsushita, S;Handa, H;Kawano, M; Interferon-? production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles Heliyon 2025-01-30 [PMID: 39897799] (Mouse)
• Hameed, M;Solomon, NA;Weger-Lucarelli, J; Lack of pathogenic involvement of CCL4 and its receptor CCR5 in arthritogenic alphavirus disease bioRxiv : the preprint server for biology 2024-08-03 [PMID: 39131287] (Mouse)
• P Selvam, CM Cheng, HU Dahms, VK Ponnusamy, YY Sun AhR Mediated Activation of Pro-Inflammatory Response of RAW 264.7 Cells Modulate the Epithelial-Mesenchymal Transition Toxics, 2022-10-27;10(11):. 2022-10-27 [PMID: 36355934] (Mouse)
• A Dietschman, S Schruefer, S Westermann, F Henkel, K Castiglion, R Willebrand, J Adam, J Ruland, R Lang, DC Sheppard, J Esser-von-, D Radtke, S Krappmann, D Voehringer Phosphatidylinositol 3-Kinase (PI3K) Orchestrates Aspergillus fumigatus-Induced Eosinophil Activation Independently of Canonical Toll-Like Receptor (TLR)/C-Type-Lectin Receptor (CLR) Signaling MBio, 2022-06-13;0(0):e0123922. 2022-06-13 [PMID: 35695427] (Mouse)
• K Carvalho, E Martin, A Ces, N Sarrazin, P Lagouge-Ro, C Nous, L Boucherit, I Youssef, A Prigent, E Faivre, S Eddarkaoui, T Gauvrit, D Vieau, S Boluda, NeuroCEB B, V Huin, B Fontaine, L Buée, B Delatour, P Dutar, F Sennlaub, X Guillonnea, D Blum, C Delarasse P2X7-deficiency improves plasticity and cognitive abilities in a mouse model of Tauopathy Progress in neurobiology, 2021-08-12;0(0):102139. 2021-08-12 [PMID: 34391810] (Mouse)
• C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting Nat. Immunol., 2020-08-31;0(0):. 2020-08-31 [PMID: 32868928] (Mouse)
• VN Camargos, G Foureaux, DC Medeiros, VT da Silveir, CM Queiroz-Ju, ALB Matosinhos, AFA Figueiredo, CDF Sousa, TP Moreira, VF Queiroz, ACF Dias, KTO Santana, I Passos, ALCV Real, LC Silva, FAG Mourão, NT Wnuk, MAP Oliveira, S Macari, T Silva, GP Garlet, JA Jackman, FM Soriani, MFD Moraes, EMAM Mendes, FM Ribeiro, GMJ Costa, AL Teixeira, NJ Cho, ACP Oliveira, MM Teixeira, VV Costa, DG Souza In-depth characterization of congenital Zika syndrome in immunocompetent mice: Antibody-dependent enhancement and an antiviral peptide therapy EBioMedicine, 2019-05-23;0(0):. 2019-05-23 [PMID: 31130472] (Mouse)
• CFCG Nunes, JS Nogueira, FB Canto, PHO Vianna, BT Ciambarell, PMRE Silva, KR Miranda, LA Lobo, RMCP Domingues, M Busch, GC Atella, AM Vale, M Bellio, A Nóbrega, R Fucs Probiotic treatment during neonatal age provides optimal protection against experimental asthma through the modulation of microbiota and T cells: Neonatal exposure to probiotics prevents asthma Int. Immunol., 2018-04-03;0(0):. 2018-04-03 [PMID: 29420746] (ELISA Capture, Mouse)
• Fevre C, Almeida A, Taront S, Pedron T, Huerre M, Prevost M, Kieusseian A, Cumano A, Brisse S, Sansonetti P, Tournebize R A novel murine model of rhinoscleroma identifies Mikulicz cells, the disease signature, as IL-10 dependent derivatives of inflammatory monocytes. EMBO Mol Med, 2013-04-01;5(4):516-30. 2013-04-01 [PMID: 23554169] (Mouse)
• Graham BS, Kines RC, Corbett KS, Nicewonger J, Roberts JN, Schiller JT, Buck CB Mucosal delivery of human papillomavirus pseudovirus-encapsidated plasmids improves the potency of DNA vaccination. Mucosal Immunol, 2010-06-16;3(5):475-86. 2010-06-16 [PMID: 20555315] (Mouse)
• Wu Y, Li YY, Matsushima K, Baba T, Mukaida N CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J. Immunol., 2008-11-01;181(9):6384-93. 2008-11-01 [PMID: 18941229] (Mouse)
• Tramonti D, Rhodes K, Martin N, Dalton JE, Andrew E, Carding SR gammadeltaT cell-mediated regulation of chemokine producing macrophages during Listeria monocytogenes infection-induced inflammation. J. Pathol., 2008-10-01;216(2):262-70. 2008-10-01 [PMID: 18767021] (Mouse)
• Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes. J. Immunol., 2007-12-15;179(12):8381-91. 2007-12-15 [PMID: 18056384] (Human)
• Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity. Immunity, 2007-04-12;26(4):445-59. 2007-04-12 [PMID: 17433730] (Mouse)
• Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses. Diabetes, 2007-03-01;56(3):809-17. 2007-03-01 [PMID: 17327452] (Mouse)
• Shiratsuchi Y, Iyoda T, Tanimoto N, Kegai D, Nagata K, Kobayashi Y Infiltrating neutrophils induce allospecific CTL in response to immunization with apoptotic cells via MCP-1 production. J. Leukoc. Biol., 2006-11-09;81(2):412-20. 2006-11-09 [PMID: 17095610] (Mouse)
• Tramonti D, Andrew EM, Rhodes K, Newton DJ, Carding SR Evidence for the opposing roles of different gamma delta T cell subsets in macrophage homeostasis. Eur. J. Immunol., 2006-07-01;36(7):1729-38. 2006-07-01 [PMID: 16783854] (Mouse)
• Tengvall S, Lundqvist A, Eisenberg RJ, Cohen GH, Harandi AM Mucosal administration of CpG oligodeoxynucleotide elicits strong CC and CXC chemokine responses in the vagina and serves as a potent Th1-tilting adjuvant for recombinant gD2 protein vaccination against genital herpes. J. Virol., 2006-06-01;80(11):5283-91. 2006-06-01 [PMID: 16699008] (Mouse)
• Punturieri A, Alviani RS, Polak T, Copper P, Sonstein J, Curtis JL Specific engagement of TLR4 or TLR3 does not lead to IFN-beta-mediated innate signal amplification and STAT1 phosphorylation in resident murine alveolar macrophages. J. Immunol., 2004-07-15;173(2):1033-42. 2004-07-15 [PMID: 15240691] (Mouse)
• Esen N, Tanga FY, DeLeo JA, Kielian T Toll-like receptor 2 (TLR2) mediates astrocyte activation in response to the Gram-positive bacterium Staphylococcus aureus. J. Neurochem., 2004-02-01;88(3):746-58. 2004-02-01 [PMID: 14720224] (Mouse)
• Raghavan S, Nystrom J, Fredriksson M, Holmgren J, Harandi AM Orally administered CpG oligodeoxynucleotide induces production of CXC and CC chemokines in the gastric mucosa and suppresses bacterial colonization in a mouse model of Helicobacter pylori infection. Infect. Immun., 2003-12-01;71(12):7014-22. 2003-12-01 [PMID: 14638791] (Mouse)

FAQs for CCL4/MIP-1 beta (DY451). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ccl4