Mouse CCL22/MDC Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse CCL22/MDC Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MDC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MDC and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MDC showed line...ar curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MDC.
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• Specificity: Natural and recombinant mouse MDC
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl22

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL22/MDC Quantikine ELISA Kit

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL22/MDC (MCC220)(23)

Publications using MCC220

• K Ming-Chin, AA Achuthan, DP De Souza, TJ Lupancu, KJ Binger, MKS Lee, Y Xu, MJ McConville, NA de Weerd, D Dragoljevi, PJ Hertzog, AJ Murphy, JA Hamilton, AJ Fleetwood Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization Cell Reports, 2022-04-19;39(3):110719. 2022-04-19 [PMID: 35443173] (Mouse)
• A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22 PloS Pathogens, 2022-01-13;18(1):e1010200. 2022-01-13 [PMID: 35025968] (Mouse)
• QR Li, SR Tan, L Yang, W He, L Chen, FX Shen, Z Wang, HF Wang Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia Journal of leukocyte biology, 2021-09-29;0(0):. 2021-09-29 [PMID: 34585429] (Mouse)
• Y Song, X Li, F Liu, H Zhu, Y Shen Isoalantolactone alleviates ovalbumin?induced asthmatic inflammation by reducing alternatively activated macrophage and STAT6/PPAR?gamma/KLF4 signals Molecular Medicine Reports, 2021-08-09;24(4):. 2021-08-09 [PMID: 34368878] (Mouse)
• KJ Warren, JA Poole, JM Sweeter, JM DeVasure, JD Dickinson, RS Peebles, TA Wyatt Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation Respiratory Research, 2021-07-15;22(1):206. 2021-07-15 [PMID: 34266437] (Mouse)
• T Yashiro, S Nakano, K Nomura, Y Uchida, K Kasakura, C Nishiyama A transcription factor PU.1 is critical for Ccl22 gene expression in dendritic cells and macrophages Sci Rep, 2019-02-04;9(1):1161. 2019-02-04 [PMID: 30718772] (Mouse)
• S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge Nat Commun, 2018-09-24;9(1):3879. 2018-09-24 [PMID: 30250029] (Mouse)
• IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality J. Exp. Med., 2018-02-14;0(0):. 2018-02-14 [PMID: 29444818] (ELISA Capture, Mouse)
• I Nishioka, T Takai, N Maruyama, S Kamijo, P Suchiva, M Suzuki, S Kunimine, H Ochi, S Shimura, K Sudo, H Ogawa, K Okumura, S Ikeda Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation J. Allergy Clin. Immunol., 2018-02-06;0(0):. 2018-02-06 [PMID: 29426602] (Mouse)
• F Wang, N Baba, Y Shen, T Yamashita, E Tsuru, M Tsuda, N Maeda, Y Sagara CCL11 promotes migration and proliferation of mouse neural progenitor cells Stem Cell Res Ther, 2017-02-07;8(1):26. 2017-02-07 [PMID: 28173860] (Mouse)
• Mishra , Amarjit, Brown , Alexandr, Yao , Xianglan, Yang , Shutong, Park , Sung-Jun, Liu , Chengyu, Dagur , Pradeep, McCoy , J Philip, Keeran , Karen J, Nugent , Gayle Z, Jeffries , Kenneth, Qu , Xuan, Yu , Zu-Xi, Levine , Stewart, Chung , Jay H Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase. Nat Commun, 2015-02-18;6(0):6224. 2015-02-18 [PMID: 25692509] (Mouse)
• Medina , Jorge L, Coalson , Jacqueli, Brooks , Edward G, Le Saux , Claude J, Winter , Vicki T, Chaparro , Adriana, Principe , Molly F, Solis , Laura, Kannan , T R, Baseman , Joel B, Dube , Peter H Mycoplasma pneumoniae CARDS toxin exacerbates ovalbumin-induced asthma-like inflammation in BALB/c mice. PLoS ONE, 2014-07-24;9(7):e102613. 2014-07-24 [PMID: 25058417] (Mouse)
• Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol, 2014-01-17;192(4):1671-80. 2014-01-17 [PMID: 24442435] (Mouse)
• Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung. J. Immunol., 2012-07-30;189(5):2545-52. 2012-07-30 [PMID: 22851704] (Mouse)
• Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets. J. Clin. Invest., 2011-08-01;121(0):3024. 2011-08-01 [PMID: 21737880] (Mouse)
• Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2. Am. J. Pathol., 2011-05-10;179(1):302-14. 2011-05-10 [PMID: 21703412] (Mouse)
• Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina. J. Immunol., 2011-01-10;186(4):2372-81. 2011-01-10 [PMID: 21220696] (Mouse)
• Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation. J. Immunol., 2009-01-01;182(1):623-35. 2009-01-01 [PMID: 19109196] (Mouse)
• Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. J. Exp. Med., 2008-08-11;205(9):2075-84. 2008-08-11 [PMID: 18695004] (Mouse)
• Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis. Am. J. Pathol., 2008-04-10;172(5):1209-21. 2008-04-10 [PMID: 18403600] (Mouse)
• Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. J. Exp. Med., 2008-03-03;205(3):699-710. 2008-03-03 [PMID: 18316417] (Mouse)
• Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity. J. Immunol., 2007-10-01;179(7):4661-71. 2007-10-01 [PMID: 17878364] (Mouse)
• Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24. 2007-02-05 [PMID: 17283337] (Mouse)

Bioinformatics

• Gene Symbol: Ccl22