Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse alpha -Fetoprotein/AFP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse AFP levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse AFP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse AFP accurately. Results obtained using natural mous...e AFP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse AFP.
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• Specificity: Natural and recombinant mouse AFP.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Afp

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for alpha-Fetoprotein/AFP (MAFP00)(17)

Publications using MAFP00

• Sun, Z;Linares, B;Urdiales, C;Al-Sarmi, F;Putluri, N;Van Nostrand, JL; AMPK activation prevents hepatocellular carcinoma development through inhibition of HNF4? activity bioRxiv : the preprint server for biology 2025-05-19 [PMID: 40475397] (Mouse)
• Chung, JY;Lee, W;Lee, OW;Ylaya, K;Nambiar, D;Sheehan-Klenk, J;Fayn, S;Hewitt, SM;Choyke, PL;Escorcia, FE; Glypican-3 deficiency in liver cancer upregulates MAPK/ERK pathway but decreases cell proliferation American journal of cancer research 2024-07-15 [PMID: 39113871] (Mouse)
• Schmithals, C;Kakoschky, B;Denk, D;von Harten, M;Klug, JH;Hintermann, E;Dropmann, A;Hamza, E;Jacomin, AC;Marquardt, JU;Zeuzem, S;Schirmacher, P;Herrmann, E;Christen, U;Vogl, TJ;Waidmann, O;Dooley, S;Finkelmeier, F;Piiper, A; Tumour-specific activation of a tumour-blood transport improves the diagnostic accuracy of blood tumour markers in mice EBioMedicine 2024-06-17 [PMID: 38889481] (Mouse)
• Zhang, Y;Liu, X;Sun, K;Luo, Y;Yang, J;Li, A;Kiupel, M;Fenske, S;Biel, M;Mi, QS;Wang, H;Xiao, H; Hyperpolarization-activated cyclic nucleotide-gated cation channel 3 promotes HCC development in a female-biased manner Cell reports 2023-09-20 [PMID: 37733590] (Mouse)
• Yasi Pan, Huarong Chen, Xiang Zhang, Weixin Liu, Yanqiang Ding, Dan Huang et al. METTL3 drives NAFLD-related hepatocellular carcinoma and is a therapeutic target for boosting immunotherapy Cell Reports Medicine 2023-08-15 [PMID: 37586322] (Mouse)
• Gerardo-Ram�rez, M;Giam, V;Becker, D;Groth, M;Hartmann, N;Morrison, H;May-Simera, HL;Radsak, MP;Marquardt, JU;Galle, PR;Herrlich, P;Straub, BK;Hartmann, M; Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice Cells 2023-04-26 [PMID: 37174657] (Mouse)
• SR Giri, B Bhoi, C Trivedi, A Rath, R Rathod, A Sharma, R Ranvir, S Kadam, K Ingale, H Patel, A Nyska, MR Jain Saroglitazar suppresses the hepatocellular carcinoma induced by intraperitoneal injection of diethylnitrosamine in C57BL/6 mice fed on choline deficient, l-amino acid- defined, high-fat diet BMC Cancer, 2023-01-17;23(1):59. 2023-01-17 [PMID: 36650455] (Mouse)
• EE Abd El-Fat, S Saber, ME Youssef, H Eissa, E El-Ahwany, NA Amin, M Alqarni, GE Batiha, AJ Obaidullah, MMY Kaddah, AG Ahmed Gaaf, AAE Mourad, G Mostafa-He, AM Abdelhamid AKT-AMPKalpha-mTOR-dependent HIF-1alpha Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma Frontiers in Pharmacology, 2022-01-12;12(0):720173. 2022-01-12 [PMID: 35095479] (Mouse)
• A Deust, MN Chobert, V Demontant, G Gricourt, T Denaës, A Thiolat, I Ruiz, C Rodriguez, JM Pawlotsky, F Teixeira-C Macrophage autophagy protects against hepatocellular carcinogenesis in mice Scientific Reports, 2021-09-22;11(1):18809. 2021-09-22 [PMID: 34552122] (Mouse)
• E Di Giorgio, L Wang, Y Xiong, T Akimova, LM Christense, R Han, A Samanta, M Trevisanut, TR Bhatti, UH Beier, WW Hancock Mef2d sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity J. Clin. Invest., 2020-12-01;0(0):. 2020-12-01 [PMID: 32790649] (Mouse)
• RB Damgaard, HE Jolin, MED Allison, SE Davies, HL Titheradge, ANJ McKenzie, D Komander OTULIN protects the liver against cell death, inflammation, fibrosis, and cancer Cell Death Differ., 2020-03-30;27(5):1457-1474. 2020-03-30 [PMID: 32231246] (Mouse)
• K Matsuura, T Takami, M Maeda, T Hisanaga, K Fujisawa, I Saeki, T Matsumoto, I Hidaka, N Yamamoto, I Sakaida Evaluation of the Effects of Cultured Bone Marrow Mesenchymal Stem Cell Infusion on Hepatocarcinogenesis in Hepatocarcinogenic Mice With Liver Cirrhosis Transplant. Proc., 2019-04-01;51(3):925-935. 2019-04-01 [PMID: 30979485] (Mouse)
• PH Jaïs, E Decroly, E Jacquet, M Le Boulch, A Jaïs, O Jean-Jean, H Eaton, P Ponien, F Verdier, B Canard, S Goncalves, S Chiron, M Le Gall, P Mayeux, M Shmulevitz C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Nucleic Acids Res., 2019-03-18;0(0):. 2019-03-18 [PMID: 30726994] (Transgenic Hamster)
• CC Fang, CF Wu, YJ Liao, SF Huang, M Chen, YA Chen AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage Sci Rep, 2018-09-14;8(1):13802. 2018-09-14 [PMID: 30217986] (Mouse)
• G Cui, RC Martin, H Jin, X Liu, H Pandit, H Zhao, L Cai, P Zhang, W Li, Y Li Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver J. Exp. Clin. Cancer Res., 2018-07-04;37(1):136. 2018-07-04 [PMID: 29973237] (Mouse)
• G Singhal, G Kumar, S Chan, FM Fisher, Y Ma, HG Vardeh, IA Nasser, JS Flier, E Maratos-Fl Deficiency of fibroblast growth factor 21 (FGF21) promotes hepatocellular carcinoma (HCC) in mice on a long term obesogenic diet Mol Metab, 2018-03-08;0(0):. 2018-03-08 [PMID: 29753678] (Mouse)
• A Ambade, A Satishchan, B Gyongyosi, P Lowe, G Szabo Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease World J Gastroenterol, 2016-04-28;22(16):4091-108. 2016-04-28 [PMID: 27122661] (Mouse)

FAQs for alpha-Fetoprotein/AFP (MAFP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Afp