Morg1 Antibody - BSA Free

Western Blot: Morg1 Antibody [NBP1-92132] - Analysis in control (vector only transfected HEK293T lysate) and WDR83 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: Morg1 Antibody [NBP1-92132] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Immunohistochemistry-Paraffin: Morg1 Antibody [NBP1-92132] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Morg1 Antibody [NBP1-92132] - Staining of human smooth muscle shows strong cytoplasmic positivity.

Analysis in control (vector only transfected HEK293T lysate) and WDR83 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Morg1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GSFDNSSLCSGGGDKAVVLWDVASGQVVRKFRGHAGKVNTVQFNEEATVILSGSIDSSIRCWDCRSRRPEPVQTLDEARDGVSSVKVSDHEIL
• Predicted Species: Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR83
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Morg1 Protein (NBP1-92132PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Morg1 Antibody - BSA Free

• MAPK organizer 1
• MGC4238
• MORG1Mitogen-activated protein kinase organizer 1
• WD repeat domain 83
• WD repeat domain-containing protein 83

Background

Mitogen-activated protein kinase organiser 1 (Morg1) is a scaffold protein for various multimeric complexes. It stabilises the assembly of several important components of the ERK pathway including Raf-1 and MEK into an oligomeric complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR83