Monoglyceride Lipase Antibody

Western Blot: Monoglyceride Lipase Antibody [NB100-96920] - Staining of Mouse Adipose lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Flow Cytometry: Monoglyceride Lipase Antibody [NB100-96920] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: Monoglyceride Lipase Antibody [NB100-96920] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NB100-96920] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Monoglyceride Lipase Antibody Summary

• Description: Novus Biologicals Goat Monoglyceride Lipase Antibody (NB100-96920) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Monoglyceride Lipase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QDLPHLVNADGQY corresponding to internal region (near N-Terminus) according to NP_001003794.1.
• Epitope: QDLPHLVNADGQY
• Specificity: This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MGLL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1).
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Monoglyceride Lipase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Monoglyceride Lipase Antibody

• HUK5
• HU-K5EC 3.1.1.23
• Lysophospholipase homolog
• Lysophospholipase-like
• MAGL
• MGLHUK5
• MGLL
• Monoacylglycerol lipase
• Monoglyceride Lipase

Background

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Monoglyceride Lipase Antibody (NB100-96920)(1)

Publication using NB100-96920

• Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol 2007-12-01 [PMID: 18096503]

Bioinformatics

• Gene Symbol: MGLL
• Entrez:
  • 11343(Human)
  • 23945(Mouse)
  • 29254(Rat)
• Uniprot:
  • Q99685()