Monoglyceride Lipase Antibody

Western Blot: Monoglyceride Lipase Antibody [NB100-2493] - Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-MGLL )

Product Discontinued

Monoglyceride Lipase Antibody Summary

• Immunogen: Immunogen corresponds to N terminal amino acids 1-121 of Human Monoacylglycerol Lipase (NP_009214.1).
• Specificity: Monoglyceride Lipase
• Clonality: Polyclonal
• Host: Chicken
• Gene: MGLL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: 1/2000. Detects a band of approximately 75 kDa for the fusion protein (predicted molecular weight for the native protein: 33 kDa).
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Monoglyceride Lipase Antibody

• HUK5
• HU-K5EC 3.1.1.23
• Lysophospholipase homolog
• Lysophospholipase-like
• MAGL
• MGLHUK5
• MGLL
• Monoacylglycerol lipase
• Monoglyceride Lipase

Background

Monoglyceride lipase is expressed in kidney, spleen, heart, liver, testis, stomach, brain and lung tissue, and adrenal gland. It is most abundantly expressed in skeletal muscle and adipose tissue. Endocannaboids such as arachidonoyl ethanolamide and 2-arachidonoyl glycerol function as short range modulators of cell and synaptic activity. Monoglyceride lipase hydrolyzes 2-arachidonoyl glycerol which terminates it's biological actions. Monoglyceride lipase also works consecutively with hormone sensitive lipase to mobilize fatty acids from triglyceride stores of adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Monoglyceride Lipase Antibody (NB100-2493)(1)

Publication using NB100-2493

• Tang ED, Wang CY. YAP-mediated induction of monoacylglycerol lipase restrains oncogenic transformation Cell. Signal. 2015-01-27 [PMID: 25636199] (WB, Human)

Bioinformatics

• Gene Symbol: MGLL
• Entrez:
  • 11343(Human)
• Uniprot:
  • Q6IBG9(Human)