Human MMRN1 ELISA Kit (Colorimetric)

ELISA: Human MMRN1 ELISA Kit (Colorimetric) [NBP3-39505] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 9.6 pg/mL (example only; lot dependent)

Human MMRN1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 9.6 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMRN1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MMRN1 ELISA Kit (Colorimetric)

• ECMmultimerin
• Elastin microfibril interface located protein 4
• Elastin microfibril interfacer 4
• EMILIN-4
• EMILIN4GPIa*
• Endothelial cell multimerin
• glycoprotein Ia*
• GPIA*
• MMRN
• multimerin 1
• multimerin-1

Background

MMRN1, also known as Multimerin-1, has 2 isoforms, a 1,228 amino acid long isoform that is 138 kDa and a short 531 amino acid isoform that is 58 kDa; synthesized by endothelial cells and megakaryocytes: stored in platelet alpha granules and endothelial cell Weibel-Palade bodies; can be found in vascular tissues such as placenta, lung, and liver. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers; it is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V and as an extracellular matrix or adhesive protein. Studies on this protein have shown a relationship with the following diseases and disorders: bleeding disorder, quebec platelet disorder, neonatal alloimmune thrombocytopenia, von Willebrand's disease, polycythemia vera, myocardial infarction, atrial fibrillation, thrombocytopenia, polycythemia, vascular disease, diabetic retinopathy, thrombosis, periodontitis, periodontal disease, hepatitis b, Parkinson's disease, vaginitis, asthma, and colorectal cancer. The MMRN1 protein has also shown an interaction with CDKN2A, F5, APC, A2M, ALB, and 30 other proteins in hemostasis, platelet degranulation, platelet activation, signaling and aggregation, and response to elevated platelet cytosolic Ca2+ pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MMRN1 ELISA Kit (NBP3-39505). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMRN1